miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1260 3' -53.4 NC_001317.1 + 15681 0.69 0.396409
Target:  5'- uGCGUGGCCGUCAUAaagaaaucgacGGCGGCGa--- -3'
miRNA:   3'- uCGUGCCGGUAGUGU-----------UCGUCGCaaua -5'
1260 3' -53.4 NC_001317.1 + 13371 0.67 0.536535
Target:  5'- cAGCGCGGCg--CACAGGcCAGCa---- -3'
miRNA:   3'- -UCGUGCCGguaGUGUUC-GUCGcaaua -5'
1260 3' -53.4 NC_001317.1 + 27365 0.67 0.548115
Target:  5'- uGGCGCGGCCGcUUGCGAcGCAuuGCGg--- -3'
miRNA:   3'- -UCGUGCCGGU-AGUGUU-CGU--CGCaaua -5'
1260 3' -53.4 NC_001317.1 + 8289 1.05 0.000945
Target:  5'- gAGCACGGCCAUCACAAGCAGCGUUAUc -3'
miRNA:   3'- -UCGUGCCGGUAGUGUUCGUCGCAAUA- -5'
1260 3' -53.4 NC_001317.1 + 16697 0.72 0.274489
Target:  5'- uGCACGGCCAgcUCACcGGCcucGCGUUu- -3'
miRNA:   3'- uCGUGCCGGU--AGUGuUCGu--CGCAAua -5'
1260 3' -53.4 NC_001317.1 + 8573 0.69 0.426702
Target:  5'- cGCACaG-CAUCAggcaGAGCAGCGUUAUa -3'
miRNA:   3'- uCGUGcCgGUAGUg---UUCGUCGCAAUA- -5'
1260 3' -53.4 NC_001317.1 + 2951 0.67 0.548115
Target:  5'- gGGCAUGGCC-UCGCAgaAGCAucuGCGc--- -3'
miRNA:   3'- -UCGUGCCGGuAGUGU--UCGU---CGCaaua -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.