Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1260 | 5' | -60.8 | NC_001317.1 | + | 8255 | 1.12 | 7.4e-05 |
Target: 5'- uUCAUGCCACCACCCCGCCAGCCGUGCg -3' miRNA: 3'- -AGUACGGUGGUGGGGCGGUCGGCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 4757 | 0.82 | 0.015907 |
Target: 5'- cUCAUcGCCACCACCgCCGCUGGCuuuCGUGCg -3' miRNA: 3'- -AGUA-CGGUGGUGG-GGCGGUCG---GCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 13426 | 0.73 | 0.085813 |
Target: 5'- gUCAUGCUgGCCugUgCGCCGcGCUGUGCa -3' miRNA: 3'- -AGUACGG-UGGugGgGCGGU-CGGCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 9859 | 0.72 | 0.111084 |
Target: 5'- ---gGCUgACCACCCgCGCCGGUCGUucGCg -3' miRNA: 3'- aguaCGG-UGGUGGG-GCGGUCGGCA--CG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 30431 | 0.71 | 0.116914 |
Target: 5'- uUUAUGCCACUuuuccacgaauuUCCCGUuuuuuUAGCCGUGCa -3' miRNA: 3'- -AGUACGGUGGu-----------GGGGCG-----GUCGGCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 14777 | 0.7 | 0.155663 |
Target: 5'- ---cGCCACCGCCCgCGCacuCGGUcugCGUGCc -3' miRNA: 3'- aguaCGGUGGUGGG-GCG---GUCG---GCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 6310 | 0.7 | 0.135395 |
Target: 5'- -aGUGCCACUuCCUCGaCCAGCauguaagguguCGUGCg -3' miRNA: 3'- agUACGGUGGuGGGGC-GGUCG-----------GCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 4240 | 0.68 | 0.210141 |
Target: 5'- aCcgGCaaauGCUACCUCGUCGGCCuUGCu -3' miRNA: 3'- aGuaCGg---UGGUGGGGCGGUCGGcACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 8506 | 0.67 | 0.239946 |
Target: 5'- ---cGCCGCUgguauaacgcuGCUCUGCCugauGCUGUGCg -3' miRNA: 3'- aguaCGGUGG-----------UGGGGCGGu---CGGCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 7617 | 0.67 | 0.252823 |
Target: 5'- aCAUGUCACUG-CCCGCCG--UGUGCg -3' miRNA: 3'- aGUACGGUGGUgGGGCGGUcgGCACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 1213 | 0.67 | 0.227621 |
Target: 5'- cUCAUGCUGCCACCUCaCCcGCUGcaacaGCg -3' miRNA: 3'- -AGUACGGUGGUGGGGcGGuCGGCa----CG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 30592 | 0.67 | 0.221661 |
Target: 5'- gCAUGaCCACUcuggaGCCgCGCCAGCUcugGCg -3' miRNA: 3'- aGUAC-GGUGG-----UGGgGCGGUCGGca-CG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 19623 | 0.67 | 0.227621 |
Target: 5'- ---cGCCGa-ACCCUGCCAGCCaaGCc -3' miRNA: 3'- aguaCGGUggUGGGGCGGUCGGcaCG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 14716 | 0.66 | 0.264894 |
Target: 5'- aUAUGCCAUCGCCUCGgaaauggucuuaCAGCCccaUGCg -3' miRNA: 3'- aGUACGGUGGUGGGGCg-----------GUCGGc--ACG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 24063 | 0.66 | 0.278138 |
Target: 5'- -gGUGCCAgCGCUugCCGuccuuaccugcgauCCAGCCGUGg -3' miRNA: 3'- agUACGGUgGUGG--GGC--------------GGUCGGCACg -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 16162 | 0.66 | 0.279563 |
Target: 5'- --cUG-CGCCACCgCCGCCAgacugacGCCGcGCa -3' miRNA: 3'- aguACgGUGGUGG-GGCGGU-------CGGCaCG- -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 19595 | 0.66 | 0.280277 |
Target: 5'- gUCAg--CACCGCCCCGCgCAGUgGUa- -3' miRNA: 3'- -AGUacgGUGGUGGGGCG-GUCGgCAcg -5' |
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1260 | 5' | -60.8 | NC_001317.1 | + | 28195 | 0.66 | 0.287501 |
Target: 5'- -uGUGCCAgCGCgCCGCCcauGCUgaaacggcggGUGCa -3' miRNA: 3'- agUACGGUgGUGgGGCGGu--CGG----------CACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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