Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12601 | 3' | -65.6 | NC_003345.1 | + | 52647 | 0.66 | 0.360208 |
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Target: 5'- --cCGCcCUCUCCGGUcGGGuuGCCc -3' miRNA: 3'- gcuGCGaGGGAGGCCAcCCCggCGGc -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 39417 | 0.67 | 0.315243 |
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Target: 5'- aCGGCGCUCCCUCgGGUGucugagucugaguGGagguacuucaucGUCGUCGu -3' miRNA: 3'- -GCUGCGAGGGAGgCCAC-------------CC------------CGGCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 7574 | 0.67 | 0.308979 |
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Target: 5'- cCGGCGUUCCauucaaCCGa--GGGCCGCCGc -3' miRNA: 3'- -GCUGCGAGGga----GGCcacCCCGGCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 35193 | 0.68 | 0.257464 |
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Target: 5'- gGGCgucgGCUCCCgugaaguccUCGGcGGGGCCGUCGu -3' miRNA: 3'- gCUG----CGAGGGa--------GGCCaCCCCGGCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 63689 | 0.69 | 0.223606 |
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Target: 5'- uCGGCGCUCUccgucaCUUCGaGUGGGGgCGCUc -3' miRNA: 3'- -GCUGCGAGG------GAGGC-CACCCCgGCGGc -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 56069 | 0.69 | 0.203195 |
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Target: 5'- gGugGCUCUCcacacggcgCUGGUGGGGgUGCUGg -3' miRNA: 3'- gCugCGAGGGa--------GGCCACCCCgGCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 10387 | 0.75 | 0.080118 |
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Target: 5'- aCGACGC-CCCa-CGGUGGGGCguuuCGCCGu -3' miRNA: 3'- -GCUGCGaGGGagGCCACCCCG----GCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 22213 | 0.78 | 0.052736 |
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Target: 5'- uCGAUGaCUUCCUCCGGgucgucaacGGGGUCGCCGu -3' miRNA: 3'- -GCUGC-GAGGGAGGCCa--------CCCCGGCGGC- -5' |
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| 12601 | 3' | -65.6 | NC_003345.1 | + | 20221 | 1.07 | 0.000278 |
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Target: 5'- aCGACGCUCCCUCCGGUGGGGCCGCCGc -3' miRNA: 3'- -GCUGCGAGGGAGGCCACCCCGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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