Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12604 | 3' | -50.7 | NC_003345.1 | + | 21884 | 1.06 | 0.005243 |
Target: 5'- cGAUGCGGUGAUUGCCGAGAUUGAGAAc -3' miRNA: 3'- -CUACGCCACUAACGGCUCUAACUCUU- -5' |
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12604 | 3' | -50.7 | NC_003345.1 | + | 34376 | 0.72 | 0.693264 |
Target: 5'- cGAUGCGGUGGauauugacguugacGCCGcGAUUGAGGGa -3' miRNA: 3'- -CUACGCCACUaa------------CGGCuCUAACUCUU- -5' |
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12604 | 3' | -50.7 | NC_003345.1 | + | 68864 | 0.67 | 0.908353 |
Target: 5'- gGGUGCGGUcGAagccguagUUGCCGuGGUcGGGAAc -3' miRNA: 3'- -CUACGCCA-CU--------AACGGCuCUAaCUCUU- -5' |
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12604 | 3' | -50.7 | NC_003345.1 | + | 54537 | 0.68 | 0.876783 |
Target: 5'- cGAUGCGaUGAUUGCCGAcgcgaagcaguauuGGUcgGAGGAc -3' miRNA: 3'- -CUACGCcACUAACGGCU--------------CUAa-CUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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