Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12605 | 3' | -57.6 | NC_003345.1 | + | 18160 | 0.73 | 0.30249 |
Target: 5'- cCGUUCGGGCcaUGAccGuuGCCGGACGaCUg -3' miRNA: 3'- -GCAAGCUCG--ACUu-CggCGGCCUGC-GG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 20465 | 0.75 | 0.261845 |
Target: 5'- gGUUCGAgagucGCUucGAGGUCGCCGGAgGUCu -3' miRNA: 3'- gCAAGCU-----CGA--CUUCGGCGGCCUgCGG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 15070 | 0.75 | 0.261845 |
Target: 5'- uGUUCgGAGC-GAAGcCCGCCGcGACuGCCg -3' miRNA: 3'- gCAAG-CUCGaCUUC-GGCGGC-CUG-CGG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 72429 | 0.84 | 0.059658 |
Target: 5'- aGUaCGcGCUGGAGCCGCCGGGCGgCg -3' miRNA: 3'- gCAaGCuCGACUUCGGCGGCCUGCgG- -5' |
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12605 | 3' | -57.6 | NC_003345.1 | + | 22719 | 1.11 | 0.000741 |
Target: 5'- uCGUUCGAGCUGAAGCCGCCGGACGCCu -3' miRNA: 3'- -GCAAGCUCGACUUCGGCGGCCUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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