Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12606 | 3' | -44.4 | NC_003345.1 | + | 75232 | 0.71 | 0.979436 |
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Target: 5'- gAGUAUAGCGUCUcCGAGGAc--GGUCa -3' miRNA: 3'- -UUAUGUUGUAGGaGCUUCUuuuCCAG- -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 64647 | 0.72 | 0.970625 |
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Target: 5'- ---cCGACGUUCUUGggGAAguuagguucGAGGUCa -3' miRNA: 3'- uuauGUUGUAGGAGCuuCUU---------UUCCAG- -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 69643 | 0.72 | 0.970625 |
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Target: 5'- --cACGGCAUcuaCCUCGAAGAcGAGGa- -3' miRNA: 3'- uuaUGUUGUA---GGAGCUUCUuUUCCag -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 63530 | 0.73 | 0.959392 |
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Target: 5'- --cGCAACAUCCUCGAugcucucucGGAGGGagcuuacccGGUCu -3' miRNA: 3'- uuaUGUUGUAGGAGCU---------UCUUUU---------CCAG- -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 14577 | 0.75 | 0.893916 |
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Target: 5'- --aGCGGCAaucuUCCUCGAAGccugcggcuaugcGGAAGGUCg -3' miRNA: 3'- uuaUGUUGU----AGGAGCUUC-------------UUUUCCAG- -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 68111 | 0.76 | 0.870505 |
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Target: 5'- -cUGCGGCAaCCUUGAGGAAAAguuuGGUCg -3' miRNA: 3'- uuAUGUUGUaGGAGCUUCUUUU----CCAG- -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 65038 | 0.78 | 0.773017 |
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Target: 5'- ---cCAGCGuaUCCUCGggGAAGGGGUg -3' miRNA: 3'- uuauGUUGU--AGGAGCuuCUUUUCCAg -5' |
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| 12606 | 3' | -44.4 | NC_003345.1 | + | 23627 | 1.1 | 0.015926 |
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Target: 5'- aAAUACAACAUCCUCGAAGAAAAGGUCg -3' miRNA: 3'- -UUAUGUUGUAGGAGCUUCUUUUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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