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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12615 | 5' | -53.2 | NC_003345.1 | + | 8519 | 0.66 | 0.886177 |
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Target: 5'- gUGCuuGAGGUCGuccaccacgAGAGCcuuUGCGAUGUu -3' miRNA: 3'- -ACGggCUCCAGC---------UCUUG---AUGCUGUAu -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 31401 | 0.67 | 0.84624 |
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Target: 5'- aGCaaCCGAGGUCGGGuuGCUACGu---- -3' miRNA: 3'- aCG--GGCUCCAGCUCu-UGAUGCuguau -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 60931 | 0.67 | 0.84624 |
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Target: 5'- gGCCUGaAGGUCGAGGucgAUUGuaacaGACAUAa -3' miRNA: 3'- aCGGGC-UCCAGCUCU---UGAUg----CUGUAU- -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 41927 | 0.67 | 0.827751 |
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Target: 5'- cGCCCGAGGUCugaauuGAguucuaccucaucGAGCgagGCGAUGUAu -3' miRNA: 3'- aCGGGCUCCAG------CU-------------CUUGa--UGCUGUAU- -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 38055 | 0.68 | 0.810245 |
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Target: 5'- aGUCCGAGGUguccgaCGAGAugUGgGugGUc -3' miRNA: 3'- aCGGGCUCCA------GCUCUugAUgCugUAu -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 7632 | 0.68 | 0.791097 |
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Target: 5'- cUGCCUGcgauucagcGGUCGAagaacgGAGCUACGGCGUc -3' miRNA: 3'- -ACGGGCu--------CCAGCU------CUUGAUGCUGUAu -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 33089 | 0.73 | 0.503677 |
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Target: 5'- cGCCgGAGGaaacCGAGAAgUACGACGg- -3' miRNA: 3'- aCGGgCUCCa---GCUCUUgAUGCUGUau -5' |
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| 12615 | 5' | -53.2 | NC_003345.1 | + | 28979 | 1.08 | 0.002912 |
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Target: 5'- aUGCCCGAGGUCGAGAACUACGACAUAg -3' miRNA: 3'- -ACGGGCUCCAGCUCUUGAUGCUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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