Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12618 | 3' | -54.6 | NC_003345.1 | + | 40360 | 0.66 | 0.873391 |
Target: 5'- gCGggCGAGUCCU-UCGuacuuGUCGGGa-- -3' miRNA: 3'- -GCaaGCUCAGGGuAGCu----CAGCCCagu -5' |
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12618 | 3' | -54.6 | NC_003345.1 | + | 9337 | 0.68 | 0.777464 |
Target: 5'- --aUCGGGaaUCUCGUCGGGUaacuccggCGGGUCGa -3' miRNA: 3'- gcaAGCUC--AGGGUAGCUCA--------GCCCAGU- -5' |
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12618 | 3' | -54.6 | NC_003345.1 | + | 16606 | 0.68 | 0.767707 |
Target: 5'- aGUaUCGAGUCCCAUCGugaacGUCGucGUUAg -3' miRNA: 3'- gCA-AGCUCAGGGUAGCu----CAGCc-CAGU- -5' |
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12618 | 3' | -54.6 | NC_003345.1 | + | 52160 | 0.71 | 0.600786 |
Target: 5'- --cUUGAGacUCCCcgcAUCGAGUCGGGUUc -3' miRNA: 3'- gcaAGCUC--AGGG---UAGCUCAGCCCAGu -5' |
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12618 | 3' | -54.6 | NC_003345.1 | + | 20767 | 0.77 | 0.29741 |
Target: 5'- gGUUCGAGUCCUucccUCGGcGUCGGGUa- -3' miRNA: 3'- gCAAGCUCAGGGu---AGCU-CAGCCCAgu -5' |
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12618 | 3' | -54.6 | NC_003345.1 | + | 32241 | 1.08 | 0.002347 |
Target: 5'- gCGUUCGAGUCCCAUCGAGUCGGGUCAc -3' miRNA: 3'- -GCAAGCUCAGGGUAGCUCAGCCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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