Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12619 | 5' | -54 | NC_003345.1 | + | 21459 | 0.71 | 0.611624 |
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Target: 5'- cAGuuGccGAgGCUCACCGGGUcgUCGCCc -3' miRNA: 3'- -UCgcCuuCUaCGAGUGGCUCA--AGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 16326 | 0.72 | 0.547378 |
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Target: 5'- gAGCGGAugauugGGAUGCUCcgauGCCGG--UCGUCg -3' miRNA: 3'- -UCGCCU------UCUACGAG----UGGCUcaAGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 52083 | 0.72 | 0.516021 |
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Target: 5'- cAGCGGGAcguuccGGUgGCUCAggauaUUGAGUUCGCCu -3' miRNA: 3'- -UCGCCUU------CUA-CGAGU-----GGCUCAAGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 70171 | 0.76 | 0.34772 |
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Target: 5'- cGCGGAAGcgugaGCUUcuuGCUGAGUUCGCUg -3' miRNA: 3'- uCGCCUUCua---CGAG---UGGCUCAAGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 48152 | 0.76 | 0.339587 |
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Target: 5'- -cCGGAAGAagaccaGCUCAUCGAGUUCGUUa -3' miRNA: 3'- ucGCCUUCUa-----CGAGUGGCUCAAGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 43947 | 0.77 | 0.271973 |
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Target: 5'- gAGCGGAAGAUucugaacaaGCUCgacgagaACCGGGaUUCGCCc -3' miRNA: 3'- -UCGCCUUCUA---------CGAG-------UGGCUC-AAGCGG- -5' |
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| 12619 | 5' | -54 | NC_003345.1 | + | 32546 | 1.13 | 0.001119 |
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Target: 5'- gAGCGGAAGAUGCUCACCGAGUUCGCCa -3' miRNA: 3'- -UCGCCUUCUACGAGUGGCUCAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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