Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12628 | 5' | -54.6 | NC_003345.1 | + | 70391 | 0.71 | 0.541197 |
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Target: 5'- aCCGACCUCGAUGaGaUgGAGGCUgACGa -3' miRNA: 3'- -GGUUGGAGCUAC-CcAgCUUCGGgUGU- -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 26851 | 0.72 | 0.520495 |
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Target: 5'- gCCGACCUCG-UGGG-CGAcauuaaaacGGCUCACc -3' miRNA: 3'- -GGUUGGAGCuACCCaGCU---------UCGGGUGu -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 15339 | 0.73 | 0.431844 |
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Target: 5'- cCCAAUcguaCUUGGUGaGGUCGggGCCgACGu -3' miRNA: 3'- -GGUUG----GAGCUAC-CCAGCuuCGGgUGU- -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 71057 | 0.74 | 0.413354 |
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Target: 5'- -uGACCUCGAUGGGUucaacgaguaCGggGUgCGCGa -3' miRNA: 3'- ggUUGGAGCUACCCA----------GCuuCGgGUGU- -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 59344 | 0.76 | 0.313314 |
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Target: 5'- uUCAACgUUGAUGGGUCGGuugacGGCCCGa- -3' miRNA: 3'- -GGUUGgAGCUACCCAGCU-----UCGGGUgu -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 46307 | 0.79 | 0.220556 |
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Target: 5'- aCCcGCCUCGAUGGuucuguccagcucGUUGAAGUCCACGg -3' miRNA: 3'- -GGuUGGAGCUACC-------------CAGCUUCGGGUGU- -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 71611 | 0.8 | 0.189249 |
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Target: 5'- cUCAACCUCG--GGGUCGAuuccAGCCCGCu -3' miRNA: 3'- -GGUUGGAGCuaCCCAGCU----UCGGGUGu -5' |
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| 12628 | 5' | -54.6 | NC_003345.1 | + | 39851 | 1.1 | 0.001564 |
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Target: 5'- uCCAACCUCGAUGGGUCGAAGCCCACAa -3' miRNA: 3'- -GGUUGGAGCUACCCAGCUUCGGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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