Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12629 | 3' | -50.9 | NC_003345.1 | + | 40743 | 1.06 | 0.005417 |
Target: 5'- gCAAAAUGGGGUGAGAAUCCCCGUGAAg -3' miRNA: 3'- -GUUUUACCCCACUCUUAGGGGCACUU- -5' |
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12629 | 3' | -50.9 | NC_003345.1 | + | 8814 | 0.7 | 0.764946 |
Target: 5'- -----gGGGGUGGGAcgaugaAUCCCCGaGAGu -3' miRNA: 3'- guuuuaCCCCACUCU------UAGGGGCaCUU- -5' |
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12629 | 3' | -50.9 | NC_003345.1 | + | 1339 | 0.67 | 0.918582 |
Target: 5'- gAAAGcGGGG-GAGAcggcCCCCGUGGu -3' miRNA: 3'- gUUUUaCCCCaCUCUua--GGGGCACUu -5' |
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12629 | 3' | -50.9 | NC_003345.1 | + | 52676 | 0.67 | 0.924671 |
Target: 5'- aGGAAUGGGGagccgucgcUGAGGAgcguucggaguUCCCCGaUGGGu -3' miRNA: 3'- gUUUUACCCC---------ACUCUU-----------AGGGGC-ACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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