Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1263 | 5' | -51.1 | NC_001317.1 | + | 15607 | 0.66 | 0.778556 |
Target: 5'- -cAGCGCcacaucAgGCAGGCcaccgACAGACCa -3' miRNA: 3'- guUUGCG------UgCGUCCGacua-UGUCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 12782 | 0.66 | 0.774235 |
Target: 5'- aGGACGCgagcacaGCGCaaaaaggcauugugAGGCUGAguagugcgGCAGACa -3' miRNA: 3'- gUUUGCG-------UGCG--------------UCCGACUa-------UGUCUGg -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 8607 | 0.66 | 0.766608 |
Target: 5'- ----gGCacugGCGCAGGCUGuucggguGUGCAG-CCg -3' miRNA: 3'- guuugCG----UGCGUCCGAC-------UAUGUCuGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 9301 | 0.66 | 0.755576 |
Target: 5'- -cGACGUGcCGCcggugacAGGCUGGcaauCAGACCg -3' miRNA: 3'- guUUGCGU-GCG-------UCCGACUau--GUCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 30218 | 0.67 | 0.734226 |
Target: 5'- -uAAUGCuuAUGguGGCgUGGUcaaACAGACCg -3' miRNA: 3'- guUUGCG--UGCguCCG-ACUA---UGUCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 4380 | 0.67 | 0.68799 |
Target: 5'- aAAGCGCugcaGCGGGUUGugcuucGCAGugCg -3' miRNA: 3'- gUUUGCGug--CGUCCGACua----UGUCugG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 5677 | 0.67 | 0.68799 |
Target: 5'- --uACGCugGCAGGUUgccGAUGCGcuucuGGCUg -3' miRNA: 3'- guuUGCGugCGUCCGA---CUAUGU-----CUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 14645 | 0.68 | 0.640752 |
Target: 5'- -cAGCGCAUG-GGGCUGua--AGACCa -3' miRNA: 3'- guUUGCGUGCgUCCGACuaugUCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 20181 | 0.68 | 0.652608 |
Target: 5'- gAAGCGCGCG-AGGgUGAgUAUAuGGCCg -3' miRNA: 3'- gUUUGCGUGCgUCCgACU-AUGU-CUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 21842 | 0.68 | 0.67624 |
Target: 5'- -uGGCGCACGguGGuCUGA----GGCCg -3' miRNA: 3'- guUUGCGUGCguCC-GACUauguCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 20658 | 0.68 | 0.664441 |
Target: 5'- --cGCGCAUGUGcGGCUGAUGgcacuuCGGACa -3' miRNA: 3'- guuUGCGUGCGU-CCGACUAU------GUCUGg -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 20244 | 0.69 | 0.568678 |
Target: 5'- --uACGCuucuaagGCGCAGGC-GAUGCGuGCCg -3' miRNA: 3'- guuUGCG-------UGCGUCCGaCUAUGUcUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 16693 | 0.7 | 0.523605 |
Target: 5'- ---cCGCGCGCAGGC----GCAGGCa -3' miRNA: 3'- guuuGCGUGCGUCCGacuaUGUCUGg -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 10468 | 0.7 | 0.523605 |
Target: 5'- --cGCGCAUGCucuGGCUGAUGuguuCAGugUc -3' miRNA: 3'- guuUGCGUGCGu--CCGACUAU----GUCugG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 15592 | 0.71 | 0.501018 |
Target: 5'- --cGCGC-CGgGGGCUGAUGCcguGCCg -3' miRNA: 3'- guuUGCGuGCgUCCGACUAUGuc-UGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 7294 | 0.72 | 0.446585 |
Target: 5'- aCGGGCGCACugaGGGCgUGGUugAGACg -3' miRNA: 3'- -GUUUGCGUGcg-UCCG-ACUAugUCUGg -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 26283 | 0.72 | 0.436095 |
Target: 5'- -uAACGCAgCGCGGGCUGGUGacu-CCu -3' miRNA: 3'- guUUGCGU-GCGUCCGACUAUgucuGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 14314 | 0.72 | 0.425748 |
Target: 5'- gCAucCGCAuCGCuGGCUGuc-CAGACCa -3' miRNA: 3'- -GUuuGCGU-GCGuCCGACuauGUCUGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 4078 | 0.73 | 0.366877 |
Target: 5'- aCAGGC-CGCGCAGGUguucaagguUGAUACGGcaACCg -3' miRNA: 3'- -GUUUGcGUGCGUCCG---------ACUAUGUC--UGG- -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 9117 | 0.76 | 0.232153 |
Target: 5'- aCGAGCGUGCGCucggugagccucAGGCUGAUACuGAUa -3' miRNA: 3'- -GUUUGCGUGCG------------UCCGACUAUGuCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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