Results 21 - 22 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1263 | 5' | -51.1 | NC_001317.1 | + | 9117 | 0.76 | 0.232153 |
Target: 5'- aCGAGCGUGCGCucggugagccucAGGCUGAUACuGAUa -3' miRNA: 3'- -GUUUGCGUGCG------------UCCGACUAUGuCUGg -5' |
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1263 | 5' | -51.1 | NC_001317.1 | + | 14667 | 1 | 0.005493 |
Target: 5'- cCAAACGCACG-AGGCUGAUACAGACCg -3' miRNA: 3'- -GUUUGCGUGCgUCCGACUAUGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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