Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12631 | 3' | -59.2 | NC_003345.1 | + | 5005 | 0.66 | 0.630881 |
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Target: 5'- gGCaGGAGCCGUgAUGGU----GGUCg -3' miRNA: 3'- gCGcCCUCGGCAgUGCCAugugCCAG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 31898 | 0.66 | 0.630881 |
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Target: 5'- -uCGGGGGCUGUUACauccGCACGGUg -3' miRNA: 3'- gcGCCCUCGGCAGUGcca-UGUGCCAg -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 77225 | 0.66 | 0.630881 |
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Target: 5'- uGUGuGGGGCCGUUguguACGGc-UAUGGUCg -3' miRNA: 3'- gCGC-CCUCGGCAG----UGCCauGUGCCAG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 63505 | 0.66 | 0.630881 |
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Target: 5'- --aGGGAGCUuaCcCGGUcuACGCGGUCg -3' miRNA: 3'- gcgCCCUCGGcaGuGCCA--UGUGCCAG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 1309 | 0.67 | 0.589456 |
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Target: 5'- aGCGGGGGCgGUUuccCGGUAUguaggcccgaaaGCGGg- -3' miRNA: 3'- gCGCCCUCGgCAGu--GCCAUG------------UGCCag -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 14605 | 0.67 | 0.57916 |
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Target: 5'- uCGUGGGAGCgGUCugGagcGUucCACGG-Cg -3' miRNA: 3'- -GCGCCCUCGgCAGugC---CAu-GUGCCaG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 46975 | 0.69 | 0.423671 |
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Target: 5'- -aCGaGGAGCCGUaCACGGUGCgagaGCuGUCg -3' miRNA: 3'- gcGC-CCUCGGCA-GUGCCAUG----UGcCAG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 75633 | 0.7 | 0.414767 |
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Target: 5'- uGCGGcGAGCuguucCGUCGCGcu-CGCGGUCu -3' miRNA: 3'- gCGCC-CUCG-----GCAGUGCcauGUGCCAG- -5' |
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| 12631 | 3' | -59.2 | NC_003345.1 | + | 45466 | 1.09 | 0.000687 |
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Target: 5'- uCGCGGGAGCCGUCACGGUACACGGUCa -3' miRNA: 3'- -GCGCCCUCGGCAGUGCCAUGUGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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