Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12632 | 5' | -54 | NC_003345.1 | + | 33036 | 0.66 | 0.880387 |
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Target: 5'- cGAGGACGuucgCC-GCGUCGUuGACa- -3' miRNA: 3'- uCUCCUGUuca-GGaCGCAGCA-CUGcu -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 56899 | 0.66 | 0.872806 |
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Target: 5'- -cGGGugGGGUaugcguaCUG-GUCGUGACGGg -3' miRNA: 3'- ucUCCugUUCAg------GACgCAGCACUGCU- -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 50425 | 0.66 | 0.869706 |
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Target: 5'- gGGAGGugGAG-CCgcuggcggagaugGCGgUgGUGACGAc -3' miRNA: 3'- -UCUCCugUUCaGGa------------CGC-AgCACUGCU- -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 23569 | 0.66 | 0.864985 |
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Target: 5'- cGAGGGCGAcggCCUGU-UCGUGACc- -3' miRNA: 3'- uCUCCUGUUca-GGACGcAGCACUGcu -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 54342 | 0.67 | 0.840149 |
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Target: 5'- cGGGGuGACAAGacagCCUGCGgcccgcucUCGgGGCGAg -3' miRNA: 3'- -UCUC-CUGUUCa---GGACGC--------AGCaCUGCU- -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 71554 | 0.67 | 0.79467 |
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Target: 5'- gGGAGGuCAAGUCCcGagaGUCGgGGCGc -3' miRNA: 3'- -UCUCCuGUUCAGGaCg--CAGCaCUGCu -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 24690 | 0.68 | 0.765344 |
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Target: 5'- cGAGGGCGAccucccggcGUCCUGCcGcCGagGACGAc -3' miRNA: 3'- uCUCCUGUU---------CAGGACG-CaGCa-CUGCU- -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 55824 | 0.7 | 0.671415 |
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Target: 5'- -aAGGugGAGUCCUcGcCGUUGUGACc- -3' miRNA: 3'- ucUCCugUUCAGGA-C-GCAGCACUGcu -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 10842 | 0.71 | 0.614244 |
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Target: 5'- cGGAGGGCGAGUCCUGCcgaacaaUCGccggguguaccuacUGGCGu -3' miRNA: 3'- -UCUCCUGUUCAGGACGc------AGC--------------ACUGCu -5' |
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| 12632 | 5' | -54 | NC_003345.1 | + | 45650 | 1.09 | 0.002074 |
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Target: 5'- cAGAGGACAAGUCCUGCGUCGUGACGAa -3' miRNA: 3'- -UCUCCUGUUCAGGACGCAGCACUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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