Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12634 | 3' | -59.7 | NC_003345.1 | + | 5687 | 0.67 | 0.502288 |
Target: 5'- aUCACCGUUaCUGCCA-GCUCCGaCGu -3' miRNA: 3'- gAGUGGCAA-GGCGGUgCGGGGCaGUu -5' |
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12634 | 3' | -59.7 | NC_003345.1 | + | 47131 | 0.68 | 0.444621 |
Target: 5'- gCUCACCGggCCggacGCCuCGCCaCCaGUCGAa -3' miRNA: 3'- -GAGUGGCaaGG----CGGuGCGG-GG-CAGUU- -5' |
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12634 | 3' | -59.7 | NC_003345.1 | + | 1219 | 0.69 | 0.382315 |
Target: 5'- uUCGCCGggCCGCCcccuguuuucCGCCCCGg--- -3' miRNA: 3'- gAGUGGCaaGGCGGu---------GCGGGGCaguu -5' |
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12634 | 3' | -59.7 | NC_003345.1 | + | 46612 | 1.05 | 0.001024 |
Target: 5'- gCUCACCGUUCCGCCACGCCCCGUCAAu -3' miRNA: 3'- -GAGUGGCAAGGCGGUGCGGGGCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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