Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12635 | 3' | -52.9 | NC_003345.1 | + | 51289 | 0.66 | 0.883792 |
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Target: 5'- --uUGuCCUCGCCACCAGagccgaaggcUCGGAUg-- -3' miRNA: 3'- ccuGC-GGAGCGGUGGUC----------AGCUUAauu -5' |
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| 12635 | 3' | -52.9 | NC_003345.1 | + | 63707 | 0.66 | 0.883792 |
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Target: 5'- --uCGCUUCGCCGCUggAGUCGGu---- -3' miRNA: 3'- ccuGCGGAGCGGUGG--UCAGCUuaauu -5' |
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| 12635 | 3' | -52.9 | NC_003345.1 | + | 20229 | 0.69 | 0.767859 |
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Target: 5'- gGGACGgaCUUGCCACggaGGUCGAAc--- -3' miRNA: 3'- -CCUGCg-GAGCGGUGg--UCAGCUUaauu -5' |
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| 12635 | 3' | -52.9 | NC_003345.1 | + | 66451 | 0.69 | 0.747367 |
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Target: 5'- -aACGCUugaaUCGCCACCGGgaCGAAUUGc -3' miRNA: 3'- ccUGCGG----AGCGGUGGUCa-GCUUAAUu -5' |
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| 12635 | 3' | -52.9 | NC_003345.1 | + | 53952 | 0.73 | 0.510162 |
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Target: 5'- -cGCGguUCUCGCCGCCGGUCGuGUUAGu -3' miRNA: 3'- ccUGC--GGAGCGGUGGUCAGCuUAAUU- -5' |
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| 12635 | 3' | -52.9 | NC_003345.1 | + | 47142 | 1.09 | 0.002612 |
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Target: 5'- cGGACGCCUCGCCACCAGUCGAAUUAAu -3' miRNA: 3'- -CCUGCGGAGCGGUGGUCAGCUUAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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