Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12635 | 5' | -57.6 | NC_003345.1 | + | 30774 | 0.66 | 0.682059 |
Target: 5'- aUUCGGggaugugUGGAaaGGcgauaagugGGGAGAGGGCGg -3' miRNA: 3'- gAAGCCa------GCCU--CCa--------CCCUCUUCCGCa -5' |
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12635 | 5' | -57.6 | NC_003345.1 | + | 18683 | 0.67 | 0.618943 |
Target: 5'- -gUCGGUgGGAuGGcaUGGGAGccGGUGg -3' miRNA: 3'- gaAGCCAgCCU-CC--ACCCUCuuCCGCa -5' |
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12635 | 5' | -57.6 | NC_003345.1 | + | 44351 | 0.69 | 0.53563 |
Target: 5'- ---aGGUgGGAaGUGGGAGggGcGCGa -3' miRNA: 3'- gaagCCAgCCUcCACCCUCuuC-CGCa -5' |
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12635 | 5' | -57.6 | NC_003345.1 | + | 55903 | 0.7 | 0.435012 |
Target: 5'- cCUUCGGugacuugacggcuaUCGGuGGUGGGGGcgguGGCGc -3' miRNA: 3'- -GAAGCC--------------AGCCuCCACCCUCuu--CCGCa -5' |
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12635 | 5' | -57.6 | NC_003345.1 | + | 56096 | 0.71 | 0.392849 |
Target: 5'- -cUCGucGUCGGuGGUGGGGGcgGAGGUGg -3' miRNA: 3'- gaAGC--CAGCCuCCACCCUC--UUCCGCa -5' |
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12635 | 5' | -57.6 | NC_003345.1 | + | 47178 | 1.08 | 0.001211 |
Target: 5'- cCUUCGGUCGGAGGUGGGAGAAGGCGUa -3' miRNA: 3'- -GAAGCCAGCCUCCACCCUCUUCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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