miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12637 3' -43.4 NC_003345.1 + 63346 0.67 0.999821
Target:  5'- -cGUGUUagaGGCGAGAGUGGAg--- -3'
miRNA:   3'- gaCGCAAgaaCCGCUCUUAUUUauau -5'
12637 3' -43.4 NC_003345.1 + 10261 0.7 0.995855
Target:  5'- -gGCGggagUGGCGAGAGUAAAc--- -3'
miRNA:   3'- gaCGCaagaACCGCUCUUAUUUauau -5'
12637 3' -43.4 NC_003345.1 + 60742 0.8 0.767229
Target:  5'- -cGCGUUCggGcGCGGGGAUGAGUAUGc -3'
miRNA:   3'- gaCGCAAGaaC-CGCUCUUAUUUAUAU- -5'
12637 3' -43.4 NC_003345.1 + 50649 1.08 0.02531
Target:  5'- cCUGCGUUCUUGGCGAGAAUAAAUAUAc -3'
miRNA:   3'- -GACGCAAGAACCGCUCUUAUUUAUAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.