miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12640 5' -50 NC_003345.1 + 67940 0.66 0.982136
Target:  5'- aUCGACCgacUCGGGCAgG-AGGUUgaUGUUCu -3'
miRNA:   3'- -AGUUGG---AGCUUGUgCaUCCAG--ACAGG- -5'
12640 5' -50 NC_003345.1 + 28852 0.66 0.974744
Target:  5'- aCGACCUCGAuUACGaaaucgccgGGGUCg--CCg -3'
miRNA:   3'- aGUUGGAGCUuGUGCa--------UCCAGacaGG- -5'
12640 5' -50 NC_003345.1 + 17497 0.67 0.965341
Target:  5'- aCAGCC-CGAACACGgcaGUCUGa-- -3'
miRNA:   3'- aGUUGGaGCUUGUGCaucCAGACagg -5'
12640 5' -50 NC_003345.1 + 42770 0.68 0.953715
Target:  5'- gUCGGCCUCGAcauucACcCGUucG-CUGUCCu -3'
miRNA:   3'- -AGUUGGAGCU-----UGuGCAucCaGACAGG- -5'
12640 5' -50 NC_003345.1 + 50399 0.69 0.928999
Target:  5'- cCAGuCUUCGAgccACACGgggagUAGGUCUGcuUCCa -3'
miRNA:   3'- aGUU-GGAGCU---UGUGC-----AUCCAGAC--AGG- -5'
12640 5' -50 NC_003345.1 + 29552 0.69 0.910855
Target:  5'- -gAACCUCGAuguuGCcuuguCGUAGGUCgauUCCa -3'
miRNA:   3'- agUUGGAGCU----UGu----GCAUCCAGac-AGG- -5'
12640 5' -50 NC_003345.1 + 51786 1.14 0.00267
Target:  5'- gUCAACCUCGAACACGUAGGUCUGUCCg -3'
miRNA:   3'- -AGUUGGAGCUUGUGCAUCCAGACAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.