Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12641 | 3' | -45.6 | NC_003345.1 | + | 36179 | 0.66 | 0.999259 |
Target: 5'- --aGGUGGaagacgaaGCUCGUCGg-GAUGUUCu -3' miRNA: 3'- cuaCUAUC--------UGAGCAGCaaCUACAAGc -5' |
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12641 | 3' | -45.6 | NC_003345.1 | + | 39785 | 0.69 | 0.990548 |
Target: 5'- ---cGUGGACUCGUCcuucggGAUGUUCa -3' miRNA: 3'- cuacUAUCUGAGCAGcaa---CUACAAGc -5' |
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12641 | 3' | -45.6 | NC_003345.1 | + | 55127 | 0.72 | 0.955413 |
Target: 5'- cGGUGAUAGGaaCGUCGUUGAUagUCa -3' miRNA: 3'- -CUACUAUCUgaGCAGCAACUAcaAGc -5' |
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12641 | 3' | -45.6 | NC_003345.1 | + | 15088 | 0.8 | 0.671634 |
Target: 5'- --cGGUGGACUCGUCGcccGUGUUCGg -3' miRNA: 3'- cuaCUAUCUGAGCAGCaacUACAAGC- -5' |
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12641 | 3' | -45.6 | NC_003345.1 | + | 51919 | 1.09 | 0.016029 |
Target: 5'- gGAUGAUAGACUCGUCGUUGAUGUUCGc -3' miRNA: 3'- -CUACUAUCUGAGCAGCAACUACAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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