Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12644 | 3' | -49.9 | NC_003345.1 | + | 33396 | 0.67 | 0.951198 |
Target: 5'- cGCUGGCgagaugaaGAAGgacGACUCCCUgAGCg -3' miRNA: 3'- aUGACUGg-------CUUCa--CUGAGGGAaUUGa -5' |
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12644 | 3' | -49.9 | NC_003345.1 | + | 63327 | 0.67 | 0.946538 |
Target: 5'- -cCUGAgCGAGcUGACUCCCggcGCUg -3' miRNA: 3'- auGACUgGCUUcACUGAGGGaauUGA- -5' |
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12644 | 3' | -49.9 | NC_003345.1 | + | 19969 | 0.68 | 0.930846 |
Target: 5'- aGCUGACCGAagaccuguacguGGUGAUUCC---GACg -3' miRNA: 3'- aUGACUGGCU------------UCACUGAGGgaaUUGa -5' |
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12644 | 3' | -49.9 | NC_003345.1 | + | 13535 | 0.68 | 0.918938 |
Target: 5'- gGCUGACCGGAGUaGCUUCgaUGAa- -3' miRNA: 3'- aUGACUGGCUUCAcUGAGGgaAUUga -5' |
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12644 | 3' | -49.9 | NC_003345.1 | + | 7086 | 0.69 | 0.89891 |
Target: 5'- ---cGACCGAAGaacGACUCCCguggugGACa -3' miRNA: 3'- augaCUGGCUUCa--CUGAGGGaa----UUGa -5' |
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12644 | 3' | -49.9 | NC_003345.1 | + | 53835 | 1.06 | 0.007167 |
Target: 5'- aUACUGACCGAAGUGACUCCCUUAACUc -3' miRNA: 3'- -AUGACUGGCUUCACUGAGGGAAUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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