miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12644 3' -49.9 NC_003345.1 + 33396 0.67 0.951198
Target:  5'- cGCUGGCgagaugaaGAAGgacGACUCCCUgAGCg -3'
miRNA:   3'- aUGACUGg-------CUUCa--CUGAGGGAaUUGa -5'
12644 3' -49.9 NC_003345.1 + 63327 0.67 0.946538
Target:  5'- -cCUGAgCGAGcUGACUCCCggcGCUg -3'
miRNA:   3'- auGACUgGCUUcACUGAGGGaauUGA- -5'
12644 3' -49.9 NC_003345.1 + 19969 0.68 0.930846
Target:  5'- aGCUGACCGAagaccuguacguGGUGAUUCC---GACg -3'
miRNA:   3'- aUGACUGGCU------------UCACUGAGGgaaUUGa -5'
12644 3' -49.9 NC_003345.1 + 13535 0.68 0.918938
Target:  5'- gGCUGACCGGAGUaGCUUCgaUGAa- -3'
miRNA:   3'- aUGACUGGCUUCAcUGAGGgaAUUga -5'
12644 3' -49.9 NC_003345.1 + 7086 0.69 0.89891
Target:  5'- ---cGACCGAAGaacGACUCCCguggugGACa -3'
miRNA:   3'- augaCUGGCUUCa--CUGAGGGaa----UUGa -5'
12644 3' -49.9 NC_003345.1 + 53835 1.06 0.007167
Target:  5'- aUACUGACCGAAGUGACUCCCUUAACUc -3'
miRNA:   3'- -AUGACUGGCUUCACUGAGGGAAUUGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.