Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12646 | 3' | -47.5 | NC_003345.1 | + | 54522 | 0.66 | 0.994442 |
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Target: 5'- ---cGACGcGA-AGCAGUAuuGGUCGGAg -3' miRNA: 3'- uuaaCUGC-CUaUUGUCGUu-CCAGUCU- -5' |
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| 12646 | 3' | -47.5 | NC_003345.1 | + | 19673 | 0.7 | 0.93745 |
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Target: 5'- ---cGACGGGcGugGGUAGGGUCAa- -3' miRNA: 3'- uuaaCUGCCUaUugUCGUUCCAGUcu -5' |
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| 12646 | 3' | -47.5 | NC_003345.1 | + | 51201 | 0.7 | 0.92596 |
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Target: 5'- --aUGAUGGAcAGCAGUuuaucguGGUCGGAa -3' miRNA: 3'- uuaACUGCCUaUUGUCGuu-----CCAGUCU- -5' |
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| 12646 | 3' | -47.5 | NC_003345.1 | + | 24509 | 0.71 | 0.899341 |
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Target: 5'- --aUGcCGGAcgaGACgAGCGAGGUCGGAc -3' miRNA: 3'- uuaACuGCCUa--UUG-UCGUUCCAGUCU- -5' |
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| 12646 | 3' | -47.5 | NC_003345.1 | + | 19055 | 0.72 | 0.88425 |
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Target: 5'- ---cGACGGGUAGCaugaagacgAGCGAGGUCu-- -3' miRNA: 3'- uuaaCUGCCUAUUG---------UCGUUCCAGucu -5' |
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| 12646 | 3' | -47.5 | NC_003345.1 | + | 54382 | 1.08 | 0.009707 |
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Target: 5'- gAAUUGACGGAUAACAGCAAGGUCAGAu -3' miRNA: 3'- -UUAACUGCCUAUUGUCGUUCCAGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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