Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12647 | 3' | -64.7 | NC_003345.1 | + | 69096 | 0.66 | 0.389079 |
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Target: 5'- gGUagUCGUCGUCCUucgcacggguCGUUCCGGGGa-- -3' miRNA: 3'- gCG--AGCGGCAGGG----------GCGAGGCCCCagu -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 5690 | 0.66 | 0.389079 |
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Target: 5'- cCGUUCGgCGUCUcguagaCCGUUCCGGGcuUCAc -3' miRNA: 3'- -GCGAGCgGCAGG------GGCGAGGCCCc-AGU- -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 49256 | 0.66 | 0.357174 |
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Target: 5'- aCGCUC-CCGaCCacguUGCUCCGGGGaugCAa -3' miRNA: 3'- -GCGAGcGGCaGGg---GCGAGGCCCCa--GU- -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 25401 | 0.68 | 0.292308 |
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Target: 5'- cCGCUaucagCGCCGUCCCgCuCUCCGaGGG-CGg -3' miRNA: 3'- -GCGA-----GCGGCAGGG-GcGAGGC-CCCaGU- -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 64292 | 0.68 | 0.285698 |
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Target: 5'- aGCgUUGUCGcCCCCGUucgggCCGGGGUgCAu -3' miRNA: 3'- gCG-AGCGGCaGGGGCGa----GGCCCCA-GU- -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 23243 | 0.73 | 0.131191 |
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Target: 5'- --gUCGCCGUCUCCGCUCgguCGGGGa-- -3' miRNA: 3'- gcgAGCGGCAGGGGCGAG---GCCCCagu -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 26585 | 0.75 | 0.091551 |
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Target: 5'- aGCUCGCCGUCaaucaUCCG-UCCGGGGUg- -3' miRNA: 3'- gCGAGCGGCAG-----GGGCgAGGCCCCAgu -5' |
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| 12647 | 3' | -64.7 | NC_003345.1 | + | 54943 | 1.08 | 0.000305 |
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Target: 5'- aCGCUCGCCGUCCCCGCUCCGGGGUCAc -3' miRNA: 3'- -GCGAGCGGCAGGGGCGAGGCCCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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