Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1265 | 5' | -55.8 | NC_001317.1 | + | 27534 | 0.66 | 0.486288 |
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Target: 5'- cGCGUGCgGCGCUUcuGCucGCGCCA-GUCg -3' miRNA: 3'- -CGUAUGaCGCGGG--UGu-CGCGGUgUAG- -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 9392 | 0.66 | 0.481984 |
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Target: 5'- gGCAUACggggugccgucggGUGCCUGCuGGCGCUugAUg -3' miRNA: 3'- -CGUAUGa------------CGCGGGUG-UCGCGGugUAg -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 1778 | 0.66 | 0.475563 |
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Target: 5'- cGCAUcgaGCaGCGCCuCACGGCuaaagaGCUGCGUUg -3' miRNA: 3'- -CGUA---UGaCGCGG-GUGUCG------CGGUGUAG- -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 12827 | 0.67 | 0.423824 |
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Target: 5'- cGCuGUGCUcGCGUCCugAGCGgUAUAUUu -3' miRNA: 3'- -CG-UAUGA-CGCGGGugUCGCgGUGUAG- -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 6139 | 0.67 | 0.423824 |
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Target: 5'- aGCAcACcgGCGaCCCACGGCGCauagaACGg- -3' miRNA: 3'- -CGUaUGa-CGC-GGGUGUCGCGg----UGUag -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 17323 | 0.68 | 0.384948 |
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Target: 5'- cCAUcgUGCGCCgguuugACAGCGCCAUAUUc -3' miRNA: 3'- cGUAugACGCGGg-----UGUCGCGGUGUAG- -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 23453 | 0.69 | 0.298595 |
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Target: 5'- aGCGUGCcGgGCUuuugCGCAGCGUCACAa- -3' miRNA: 3'- -CGUAUGaCgCGG----GUGUCGCGGUGUag -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 14771 | 0.7 | 0.290835 |
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Target: 5'- gGCAUACgccacCGCCCGCGcacucggucugcGUGCCuACAUCg -3' miRNA: 3'- -CGUAUGac---GCGGGUGU------------CGCGG-UGUAG- -5' |
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| 1265 | 5' | -55.8 | NC_001317.1 | + | 15622 | 1.12 | 0.000202 |
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Target: 5'- gGCAUACUGCGCCCACAGCGCCACAUCa -3' miRNA: 3'- -CGUAUGACGCGGGUGUCGCGGUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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