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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12651 | 3' | -51.1 | NC_003345.1 | + | 58522 | 1.07 | 0.005074 |
Target: 5'- aGUCCAUCGUGAGAGGACGAAUCUCAAg -3' miRNA: 3'- -CAGGUAGCACUCUCCUGCUUAGAGUU- -5' |
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12651 | 3' | -51.1 | NC_003345.1 | + | 47104 | 0.71 | 0.744244 |
Target: 5'- gGUUCAUCGUGGcgaagcgaaacGAGGGCGAGUUUgCGAa -3' miRNA: 3'- -CAGGUAGCACU-----------CUCCUGCUUAGA-GUU- -5' |
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12651 | 3' | -51.1 | NC_003345.1 | + | 3283 | 0.75 | 0.520885 |
Target: 5'- cUCCGgcucucucguuggcUCGUGAuGGGGAUGAAUCUCAc -3' miRNA: 3'- cAGGU--------------AGCACU-CUCCUGCUUAGAGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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