Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12652 | 3' | -56.2 | NC_003345.1 | + | 65159 | 0.66 | 0.742264 |
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Target: 5'- aACGUCCCCACU--UC-CGUcAGCuCUCc -3' miRNA: 3'- -UGCAGGGGUGGgaAGuGCA-UCGuGAG- -5' |
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| 12652 | 3' | -56.2 | NC_003345.1 | + | 15141 | 0.66 | 0.732024 |
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Target: 5'- gGCGuUCCCCAaguccagcuCCCga-ACGUAGUAgUCg -3' miRNA: 3'- -UGC-AGGGGU---------GGGaagUGCAUCGUgAG- -5' |
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| 12652 | 3' | -56.2 | NC_003345.1 | + | 45742 | 0.67 | 0.690221 |
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Target: 5'- aGCGUCUCCGCuuccuCCUUCACGgcGUccACaUCg -3' miRNA: 3'- -UGCAGGGGUG-----GGAAGUGCauCG--UG-AG- -5' |
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| 12652 | 3' | -56.2 | NC_003345.1 | + | 76897 | 0.68 | 0.615397 |
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Target: 5'- aGCGaaCCCgGCCC-UCGCGUGucguGCGCUCc -3' miRNA: 3'- -UGCa-GGGgUGGGaAGUGCAU----CGUGAG- -5' |
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| 12652 | 3' | -56.2 | NC_003345.1 | + | 48059 | 0.72 | 0.39536 |
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Target: 5'- ---aCCCCACCCcucgUgGCGUgGGCACUCg -3' miRNA: 3'- ugcaGGGGUGGGa---AgUGCA-UCGUGAG- -5' |
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| 12652 | 3' | -56.2 | NC_003345.1 | + | 58747 | 1.08 | 0.001392 |
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Target: 5'- aACGUCCCCACCCUUCACGUAGCACUCc -3' miRNA: 3'- -UGCAGGGGUGGGAAGUGCAUCGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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