Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12656 | 3' | -59.3 | NC_003345.1 | + | 21172 | 0.66 | 0.620672 |
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Target: 5'- aGCGGuCGCCCGGcgACgaGUCGGaUGAUa -3' miRNA: 3'- gCGCUcGCGGGCCa-UG--UAGCCgACUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 68648 | 0.66 | 0.620672 |
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Target: 5'- uGCGAGCGCaucCCGGUcgcCAUCGG--GAUg -3' miRNA: 3'- gCGCUCGCG---GGCCAu--GUAGCCgaCUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 23984 | 0.66 | 0.610225 |
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Target: 5'- cCGUgGAGCGUCCGGccccACGUCGG--GACa -3' miRNA: 3'- -GCG-CUCGCGGGCCa---UGUAGCCgaCUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 63505 | 0.66 | 0.589388 |
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Target: 5'- aGgGAGCuuaCCCGGUcuacGCGgucgCGGUUGACc -3' miRNA: 3'- gCgCUCGc--GGGCCA----UGUa---GCCGACUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 63236 | 0.66 | 0.586273 |
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Target: 5'- -aCGGGCGaCUCGGUggacuACGcuacugccgacuacUCGGCUGGCa -3' miRNA: 3'- gcGCUCGC-GGGCCA-----UGU--------------AGCCGACUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 51243 | 0.67 | 0.54816 |
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Target: 5'- aCGCGAGCGUCCaa---GUCGG-UGACg -3' miRNA: 3'- -GCGCUCGCGGGccaugUAGCCgACUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 24646 | 0.68 | 0.498002 |
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Target: 5'- aGCGAGCGCCgUGGU---UCGGUgaaaGACc -3' miRNA: 3'- gCGCUCGCGG-GCCAuguAGCCGa---CUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 14453 | 0.71 | 0.338417 |
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Target: 5'- gCGUGAGUaacuacccaCCGGgaACGUCGGCUGGCg -3' miRNA: 3'- -GCGCUCGcg-------GGCCa-UGUAGCCGACUG- -5' |
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| 12656 | 3' | -59.3 | NC_003345.1 | + | 59630 | 1.09 | 0.000614 |
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Target: 5'- aCGCGAGCGCCCGGUACAUCGGCUGACu -3' miRNA: 3'- -GCGCUCGCGGGCCAUGUAGCCGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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