Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12657 | 3' | -56.8 | NC_003345.1 | + | 73720 | 0.66 | 0.734916 |
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Target: 5'- cGCGgagCCGCUgACGACUacuccuaCGGCCucagGCg -3' miRNA: 3'- uCGCaa-GGCGG-UGCUGA-------GCCGGua--UG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 37606 | 0.66 | 0.70496 |
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Target: 5'- uGcCGgaCUGUCACggggGACUCGGCCcgGCg -3' miRNA: 3'- uC-GCaaGGCGGUG----CUGAGCCGGuaUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 35678 | 0.67 | 0.673382 |
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Target: 5'- gGGCG-UCCuCCACGgacguaGCUCGGUCGgGCg -3' miRNA: 3'- -UCGCaAGGcGGUGC------UGAGCCGGUaUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 52156 | 0.67 | 0.662769 |
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Target: 5'- gGGCaUUa-GCCACGACUCGGCaa-ACg -3' miRNA: 3'- -UCGcAAggCGGUGCUGAGCCGguaUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 26064 | 0.68 | 0.609492 |
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Target: 5'- uGUGUUCUGCCGCG-UUCuGaCCGUGCc -3' miRNA: 3'- uCGCAAGGCGGUGCuGAGcC-GGUAUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 15064 | 0.68 | 0.598856 |
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Target: 5'- gAGCGaagcCCGCCGCGACU--GCCGgGCa -3' miRNA: 3'- -UCGCaa--GGCGGUGCUGAgcCGGUaUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 31795 | 0.68 | 0.598856 |
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Target: 5'- cGGUGUuuUCCGgUGCGACUC-GCUGUACg -3' miRNA: 3'- -UCGCA--AGGCgGUGCUGAGcCGGUAUG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 23861 | 0.7 | 0.505225 |
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Target: 5'- gAGCGUUCCGCCagcgaACGACugcugagcgUCGGCa---- -3' miRNA: 3'- -UCGCAAGGCGG-----UGCUG---------AGCCGguaug -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 45705 | 0.71 | 0.409367 |
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Target: 5'- gAGCGUUCCaagaguuCUGCGACUCGGgCAUcaGCa -3' miRNA: 3'- -UCGCAAGGc------GGUGCUGAGCCgGUA--UG- -5' |
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| 12657 | 3' | -56.8 | NC_003345.1 | + | 60124 | 1.09 | 0.001033 |
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Target: 5'- gAGCGUUCCGCCACGACUCGGCCAUACu -3' miRNA: 3'- -UCGCAAGGCGGUGCUGAGCCGGUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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