Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12658 | 5' | -53.3 | NC_003345.1 | + | 70648 | 0.66 | 0.881626 |
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Target: 5'- cGUgAGgcGCuuCACCACUUCGUCGUgGGu -3' miRNA: 3'- -UAgUCaaCG--GUGGUGAAGCGGUAgCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 20233 | 0.66 | 0.873917 |
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Target: 5'- -cCGGUgggGCCGCCGCUgUCuCCuUCGAu -3' miRNA: 3'- uaGUCAa--CGGUGGUGA-AGcGGuAGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 73428 | 0.66 | 0.873917 |
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Target: 5'- -aCAGggGuuCCACgACgaggUUCGCCGUCGAa -3' miRNA: 3'- uaGUCaaC--GGUGgUG----AAGCGGUAGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 50331 | 0.67 | 0.794166 |
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Target: 5'- -cCAGcgGCucCACCucccgauacgaGCUUCGCCAUCGGg -3' miRNA: 3'- uaGUCaaCG--GUGG-----------UGAAGCGGUAGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 5655 | 0.68 | 0.753869 |
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Target: 5'- -gUAGUcgucguccucUGCCAUCACUUCGUCAaccgccauuUCGAg -3' miRNA: 3'- uaGUCA----------ACGGUGGUGAAGCGGU---------AGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 2719 | 0.7 | 0.645997 |
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Target: 5'- uUCGG-UGUCACUACcgccgUCGCCGUCGc -3' miRNA: 3'- uAGUCaACGGUGGUGa----AGCGGUAGCu -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 48943 | 0.7 | 0.645997 |
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Target: 5'- uUguGUUGCCACCGauugUCGCgguaCAUCGAc -3' miRNA: 3'- uAguCAACGGUGGUga--AGCG----GUAGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 29482 | 0.72 | 0.525972 |
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Target: 5'- -gCAGUcgGCguCCACUUUGUCGUCGAu -3' miRNA: 3'- uaGUCAa-CGguGGUGAAGCGGUAGCU- -5' |
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| 12658 | 5' | -53.3 | NC_003345.1 | + | 60275 | 1.07 | 0.002644 |
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Target: 5'- aAUCAGUUGCCACCACUUCGCCAUCGAg -3' miRNA: 3'- -UAGUCAACGGUGGUGAAGCGGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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