Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1266 | 3' | -50.1 | NC_001317.1 | + | 3488 | 0.66 | 0.802108 |
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Target: 5'- gUCACCGCCugacuuuugcgGCUUg--GCGAUGAgcUGAa -3' miRNA: 3'- -AGUGGCGG-----------CGAAaagCGUUACU--ACUa -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 8241 | 0.66 | 0.802108 |
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Target: 5'- cCGCCaGCCGUg---CGCcaGAUGGUGAc -3' miRNA: 3'- aGUGG-CGGCGaaaaGCG--UUACUACUa -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 13193 | 0.67 | 0.7471 |
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Target: 5'- aUCACCGCCGCcgucaUCGCcauuacUGAUGc- -3' miRNA: 3'- -AGUGGCGGCGaaa--AGCGuu----ACUACua -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 16440 | 0.69 | 0.604293 |
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Target: 5'- aCGCUGCagCGcCUUUUCGCGccauGUGAUGAg -3' miRNA: 3'- aGUGGCG--GC-GAAAAGCGU----UACUACUa -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 4747 | 0.7 | 0.551736 |
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Target: 5'- cCACCGCCGCUggcuuucgugcgUCGCGGcUGGcUGAa -3' miRNA: 3'- aGUGGCGGCGAaa----------AGCGUU-ACU-ACUa -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 12238 | 0.72 | 0.431459 |
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Target: 5'- cCGCUGCCaGUUUUUCggcuccgGCAGUGGUGAUa -3' miRNA: 3'- aGUGGCGG-CGAAAAG-------CGUUACUACUA- -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 5726 | 0.73 | 0.38169 |
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Target: 5'- cCACCGCCGCa----GCAuUGAUGAa -3' miRNA: 3'- aGUGGCGGCGaaaagCGUuACUACUa -5' |
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| 1266 | 3' | -50.1 | NC_001317.1 | + | 17256 | 1.06 | 0.002063 |
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Target: 5'- aUCACCGCCGCUUUUCGCAAUGAUGAUu -3' miRNA: 3'- -AGUGGCGGCGAAAAGCGUUACUACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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