Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12661 | 5' | -53.1 | NC_003345.1 | + | 42487 | 0.67 | 0.862752 |
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Target: 5'- uGAUuuUugUGACcUCGCCCGucccCUUCGCUa -3' miRNA: 3'- -CUGggA--ACUGaAGCGGGUu---GAAGCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 16065 | 0.67 | 0.862752 |
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Target: 5'- uGCuCCUUGGg-UUGCCUAACUcUGCCg -3' miRNA: 3'- cUG-GGAACUgaAGCGGGUUGAaGCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 73401 | 0.67 | 0.85455 |
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Target: 5'- aGACCUgaGAgaUUCGCUCAGCcacCGCCg -3' miRNA: 3'- -CUGGGaaCUg-AAGCGGGUUGaa-GCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 24447 | 0.67 | 0.837467 |
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Target: 5'- cGCCUgcGAUUUCGUCCAguugaGCUaucaucuggUCGCCg -3' miRNA: 3'- cUGGGaaCUGAAGCGGGU-----UGA---------AGCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 24156 | 0.71 | 0.633544 |
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Target: 5'- uGACCUUcUGAUUcgCGUCCAGCUugUCGCUg -3' miRNA: 3'- -CUGGGA-ACUGAa-GCGGGUUGA--AGCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 24381 | 0.72 | 0.557649 |
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Target: 5'- cGACCCguggguugacgUUGAC-UCGUCCGACcUCGCUc -3' miRNA: 3'- -CUGGG-----------AACUGaAGCGGGUUGaAGCGG- -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 15241 | 0.81 | 0.188128 |
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Target: 5'- cGACCCUUGACuUUCGCUCGGCggCGUa -3' miRNA: 3'- -CUGGGAACUG-AAGCGGGUUGaaGCGg -5' |
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| 12661 | 5' | -53.1 | NC_003345.1 | + | 60893 | 1.13 | 0.001427 |
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Target: 5'- uGACCCUUGACUUCGCCCAACUUCGCCa -3' miRNA: 3'- -CUGGGAACUGAAGCGGGUUGAAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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