Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12664 | 3' | -56.5 | NC_003345.1 | + | 27677 | 0.66 | 0.747189 |
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Target: 5'- uGGCGCUGuucaaacUGGCCcgAGCGCAGGGc -3' miRNA: 3'- gUCGCGGCugcu---AUCGG--UCGCGUUCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 63203 | 0.66 | 0.736977 |
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Target: 5'- gCGGgGuuGACGuagaccucGCCAGCGcCGGGAg -3' miRNA: 3'- -GUCgCggCUGCuau-----CGGUCGC-GUUCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 36816 | 0.66 | 0.736977 |
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Target: 5'- --aCGCCGugG-UAGCCGGuCGUcGGAa -3' miRNA: 3'- gucGCGGCugCuAUCGGUC-GCGuUCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 41995 | 0.66 | 0.716263 |
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Target: 5'- uCAGCGCCGuuAUGA-GGUCAGCGaauGGGc -3' miRNA: 3'- -GUCGCGGC--UGCUaUCGGUCGCgu-UCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 50123 | 0.67 | 0.684622 |
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Target: 5'- gCGGCuCCGGCGGUGGaggUGGCGguGGAg -3' miRNA: 3'- -GUCGcGGCUGCUAUCg--GUCGCguUCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 14580 | 0.68 | 0.577512 |
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Target: 5'- aCGGCGCuCGugGGUcGCCAGC-CGAc- -3' miRNA: 3'- -GUCGCG-GCugCUAuCGGUCGcGUUcu -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 32800 | 0.69 | 0.525021 |
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Target: 5'- gGGcCGCCGuagcCGGUGuagguguuGCCGGUGCGAGAg -3' miRNA: 3'- gUC-GCGGCu---GCUAU--------CGGUCGCGUUCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 61148 | 0.73 | 0.347744 |
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Target: 5'- gAGCGUCGGCGGcgguaaUGGUCGGCGCucucGGAg -3' miRNA: 3'- gUCGCGGCUGCU------AUCGGUCGCGu---UCU- -5' |
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| 12664 | 3' | -56.5 | NC_003345.1 | + | 62969 | 1.09 | 0.001101 |
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Target: 5'- aCAGCGCCGACGAUAGCCAGCGCAAGAu -3' miRNA: 3'- -GUCGCGGCUGCUAUCGGUCGCGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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