Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1267 | 5' | -57.5 | NC_001317.1 | + | 18086 | 1.06 | 0.000374 |
Target: 5'- uAGAGCGCCACCGGCGAUGAUGCCAAUg -3' miRNA: 3'- -UCUCGCGGUGGCCGCUACUACGGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 2749 | 0.8 | 0.035398 |
Target: 5'- cAGAGCugGCCACCGGCGAgcgcgcggugacUGAUGUCAAUu -3' miRNA: 3'- -UCUCG--CGGUGGCCGCU------------ACUACGGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 2472 | 0.72 | 0.153758 |
Target: 5'- --cGCGCCGCCGGUGGuuucUGGUGgCAAg -3' miRNA: 3'- ucuCGCGGUGGCCGCU----ACUACgGUUa -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 5097 | 0.72 | 0.145145 |
Target: 5'- -aGGUGUCggucguGCUGGCGAUGGUGCCGGUc -3' miRNA: 3'- ucUCGCGG------UGGCCGCUACUACGGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 9223 | 0.71 | 0.182435 |
Target: 5'- aGGAGCGCCucGCCGGUc-UGAUuGCCAGc -3' miRNA: 3'- -UCUCGCGG--UGGCCGcuACUA-CGGUUa -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 4700 | 0.7 | 0.193 |
Target: 5'- cGAaaGCCAgCGGCGGUGGUGgCGAUg -3' miRNA: 3'- uCUcgCGGUgGCCGCUACUACgGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 28809 | 0.7 | 0.215744 |
Target: 5'- cAGGGCuucGCC-CCGGCuGAuugUGAUGCCGGUu -3' miRNA: 3'- -UCUCG---CGGuGGCCG-CU---ACUACGGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 15578 | 0.69 | 0.247363 |
Target: 5'- uGGGCGCaguaugccgCGCCGGgGGcUGAUGCCGu- -3' miRNA: 3'- uCUCGCG---------GUGGCCgCU-ACUACGGUua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 7092 | 0.69 | 0.247363 |
Target: 5'- uGAGCGCUAUcgCGGCGuUGAgGCCAc- -3' miRNA: 3'- uCUCGCGGUG--GCCGCuACUaCGGUua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 6682 | 0.69 | 0.254129 |
Target: 5'- gGGAGCGUUcguuaACCGGCGGUuuuUGCCGc- -3' miRNA: 3'- -UCUCGCGG-----UGGCCGCUAcu-ACGGUua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 2434 | 0.68 | 0.29795 |
Target: 5'- uGGAGCGCCACCaguggaagGGC-AUGGacuuUGCCGc- -3' miRNA: 3'- -UCUCGCGGUGG--------CCGcUACU----ACGGUua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 9375 | 0.67 | 0.33877 |
Target: 5'- cGGGUGCCuGCUGGCGcuUGAUGCg--- -3' miRNA: 3'- uCUCGCGG-UGGCCGCu-ACUACGguua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 7694 | 0.66 | 0.390069 |
Target: 5'- -cAGCGCCGCUuuaagucacaaaagGGgGAaagUGAUGCCGGUc -3' miRNA: 3'- ucUCGCGGUGG--------------CCgCU---ACUACGGUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 612 | 0.66 | 0.377931 |
Target: 5'- cAGAGCGCgACCGacgucgaaucgcugcGCGAggcGAUGCgCAAc -3' miRNA: 3'- -UCUCGCGgUGGC---------------CGCUa--CUACG-GUUa -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 19099 | 0.66 | 0.383501 |
Target: 5'- uGAGauccaGCCGCCGGUGAUGGcUGgguaCAGUg -3' miRNA: 3'- uCUCg----CGGUGGCCGCUACU-ACg---GUUA- -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 14825 | 0.66 | 0.392906 |
Target: 5'- cGAGUG-CGCgGGCGGUGGcguaUGCCGu- -3' miRNA: 3'- uCUCGCgGUGgCCGCUACU----ACGGUua -5' |
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1267 | 5' | -57.5 | NC_001317.1 | + | 18740 | 0.66 | 0.40246 |
Target: 5'- aAGAGCGucCCGCCGGUGcgGuaacugGCUggUa -3' miRNA: 3'- -UCUCGC--GGUGGCCGCuaCua----CGGuuA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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