Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12670 | 3' | -57.7 | NC_003345.1 | + | 21839 | 0.66 | 0.696868 |
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Target: 5'- gGAAGccCCUCGUCgucaCCGCC-GGAGUCGc -3' miRNA: 3'- -CUUCa-GGAGCGG----GGCGGaCUUCAGCa -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 69951 | 0.66 | 0.686402 |
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Target: 5'- cGAAGUUC-CGCUCCGCUUcGucGUUGUg -3' miRNA: 3'- -CUUCAGGaGCGGGGCGGA-CuuCAGCA- -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 63687 | 0.66 | 0.680099 |
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Target: 5'- uGAGUCaacgaccgauucaaUCGCUUCGCCgcUGGAGUCGg -3' miRNA: 3'- cUUCAGg-------------AGCGGGGCGG--ACUUCAGCa -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 52630 | 0.66 | 0.675889 |
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Target: 5'- uAAGUCCUCcuuuuGUCCCGCCcucuccGGUCGg -3' miRNA: 3'- cUUCAGGAG-----CGGGGCGGacu---UCAGCa -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 73594 | 0.67 | 0.622931 |
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Target: 5'- gGGAGUa-UCGUaCCGCCUGAGGcCGUa -3' miRNA: 3'- -CUUCAggAGCGgGGCGGACUUCaGCA- -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 63204 | 0.67 | 0.601738 |
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Target: 5'- cGggGUugacguagaCCUCGCCagCGCCgGGAGUCa- -3' miRNA: 3'- -CuuCA---------GGAGCGGg-GCGGaCUUCAGca -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 75476 | 0.67 | 0.580641 |
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Target: 5'- ---uUCCUcCGCCCaCGUCUGAacGGUCGc -3' miRNA: 3'- cuucAGGA-GCGGG-GCGGACU--UCAGCa -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 36022 | 0.68 | 0.53898 |
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Target: 5'- gGAAGUCCUCGUagaGCCgggaaGGAGUCGc -3' miRNA: 3'- -CUUCAGGAGCGgggCGGa----CUUCAGCa -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 63366 | 0.68 | 0.53898 |
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Target: 5'- aGAGGUCUUCGCCCguaaCGUCcacGAGGUCa- -3' miRNA: 3'- -CUUCAGGAGCGGG----GCGGa--CUUCAGca -5' |
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| 12670 | 3' | -57.7 | NC_003345.1 | + | 65860 | 0.96 | 0.007631 |
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Target: 5'- uGAAGUCCU-GCCCCGCCUGAAGUCGUa -3' miRNA: 3'- -CUUCAGGAgCGGGGCGGACUUCAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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