Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12670 | 5' | -49.4 | NC_003345.1 | + | 51722 | 0.66 | 0.978738 |
Target: 5'- cGugGAUaaUGUCCAcaccggaGCGAGAGAGUa- -3' miRNA: 3'- cUugCUGa-ACAGGU-------UGCUCUCUCAca -5' |
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12670 | 5' | -49.4 | NC_003345.1 | + | 74965 | 0.67 | 0.959911 |
Target: 5'- aGAAuCGACg---CCAGCGGGAGAGa-- -3' miRNA: 3'- -CUU-GCUGaacaGGUUGCUCUCUCaca -5' |
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12670 | 5' | -49.4 | NC_003345.1 | + | 44671 | 0.71 | 0.852694 |
Target: 5'- gGAGCGACag--UCAGCGGGAGAGUa- -3' miRNA: 3'- -CUUGCUGaacaGGUUGCUCUCUCAca -5' |
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12670 | 5' | -49.4 | NC_003345.1 | + | 62791 | 0.72 | 0.810721 |
Target: 5'- cGAGCGACUUGaCgGACGAGAGGcgcuuguugauuggcGUGg -3' miRNA: 3'- -CUUGCUGAACaGgUUGCUCUCU---------------CACa -5' |
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12670 | 5' | -49.4 | NC_003345.1 | + | 38044 | 0.73 | 0.745731 |
Target: 5'- cGAGCuGGCUgaguccgaggUGUCCGACGAGAuguGGGUGg -3' miRNA: 3'- -CUUG-CUGA----------ACAGGUUGCUCU---CUCACa -5' |
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12670 | 5' | -49.4 | NC_003345.1 | + | 65895 | 1.09 | 0.00599 |
Target: 5'- cGAACGACUUGUCCAACGAGAGAGUGUa -3' miRNA: 3'- -CUUGCUGAACAGGUUGCUCUCUCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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