Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12673 | 3' | -55.9 | NC_003345.1 | + | 51824 | 0.66 | 0.750067 |
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Target: 5'- -aCGgUCAGACUauuauCAGCGgUCGCCCg -3' miRNA: 3'- aaGCgAGUCUGGgu---GUUGC-AGUGGGa -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 19342 | 0.66 | 0.728412 |
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Target: 5'- gUCGCUCAGuccguucguaacaACCCGgAAC-UCAUCCa -3' miRNA: 3'- aAGCGAGUC-------------UGGGUgUUGcAGUGGGa -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 63316 | 0.67 | 0.676459 |
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Target: 5'- cUCGCUCAccGGCCCcucggcCAGCGU-GCCCg -3' miRNA: 3'- aAGCGAGU--CUGGGu-----GUUGCAgUGGGa -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 38378 | 0.68 | 0.611624 |
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Target: 5'- cUCGCUCAGACUUACG-CGagcuucaaUCGCCUUc -3' miRNA: 3'- aAGCGAGUCUGGGUGUuGC--------AGUGGGA- -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 70761 | 0.69 | 0.547378 |
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Target: 5'- -gCGCUCcGACCCACGACGaaguggugaagCGCCUc -3' miRNA: 3'- aaGCGAGuCUGGGUGUUGCa----------GUGGGa -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 49181 | 0.7 | 0.516021 |
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Target: 5'- cUCGCUUAGAagaaCCCACGACcccUCGCUCUu -3' miRNA: 3'- aAGCGAGUCU----GGGUGUUGc--AGUGGGA- -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 61390 | 0.78 | 0.161989 |
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Target: 5'- cUCGUcuUCaAGACCCuCGGCGUCACCCUg -3' miRNA: 3'- aAGCG--AG-UCUGGGuGUUGCAGUGGGA- -5' |
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| 12673 | 3' | -55.9 | NC_003345.1 | + | 69770 | 1.05 | 0.002403 |
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Target: 5'- cUUCGCUCAGACCCACAACGUCACCCUc -3' miRNA: 3'- -AAGCGAGUCUGGGUGUUGCAGUGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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