miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12673 5' -57.7 NC_003345.1 + 21623 0.66 0.700518
Target:  5'- aGGAGAggaugacacgGCGGcCUUCGaGGUCGucacGGAGUUc -3'
miRNA:   3'- -CCUCU----------CGCC-GAAGCcCCAGC----UCUCAA- -5'
12673 5' -57.7 NC_003345.1 + 20461 0.66 0.690087
Target:  5'- cGAGAGUcGCUUCGaGGUCGccGGAGg- -3'
miRNA:   3'- cCUCUCGcCGAAGCcCCAGC--UCUCaa -5'
12673 5' -57.7 NC_003345.1 + 8215 0.66 0.658521
Target:  5'- uGGAGA--GGCUUCGGGucGUCGGGGu-- -3'
miRNA:   3'- -CCUCUcgCCGAAGCCC--CAGCUCUcaa -5'
12673 5' -57.7 NC_003345.1 + 50344 0.67 0.605576
Target:  5'- uGGAGgugcuGGCGGCUccuUCGGGGgucgCGGuGGUa -3'
miRNA:   3'- -CCUC-----UCGCCGA---AGCCCCa---GCUcUCAa -5'
12673 5' -57.7 NC_003345.1 + 69805 1.06 0.001343
Target:  5'- gGGAGAGCGGCUUCGGGGUCGAGAGUUu -3'
miRNA:   3'- -CCUCUCGCCGAAGCCCCAGCUCUCAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.