Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12675 | 3' | -57.3 | NC_003345.1 | + | 75308 | 0.66 | 0.707276 |
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Target: 5'- uGAACCgCCGAccuCGCGGCCC-CGGu- -3' miRNA: 3'- -CUUGGaGGCUcu-GCGCCGGGaGUUcu -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 61678 | 0.66 | 0.695824 |
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Target: 5'- --cCCUCUGAGAgccaguuCGCGGagCUUCGAGAu -3' miRNA: 3'- cuuGGAGGCUCU-------GCGCCg-GGAGUUCU- -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 43131 | 0.66 | 0.686402 |
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Target: 5'- cAACUUCgGGGAauacuuCGCGGCUCUaCAAGGc -3' miRNA: 3'- cUUGGAGgCUCU------GCGCCGGGA-GUUCU- -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 58831 | 0.66 | 0.675889 |
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Target: 5'- aAGCgUCUGA-ACGCGGCggagauuuuCCUCAAGAa -3' miRNA: 3'- cUUGgAGGCUcUGCGCCG---------GGAGUUCU- -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 60175 | 0.66 | 0.675889 |
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Target: 5'- aGACCUguaCGAGACGUuccaGCCCUCGAu- -3' miRNA: 3'- cUUGGAg--GCUCUGCGc---CGGGAGUUcu -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 60413 | 0.67 | 0.633543 |
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Target: 5'- cGAACCUUCGAGgcauuauccguaGCGCGauuCCCUCGAu- -3' miRNA: 3'- -CUUGGAGGCUC------------UGCGCc--GGGAGUUcu -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 56020 | 0.67 | 0.62187 |
|
Target: 5'- aGACCUCCuauccaguuucggGAGACGUGaCCCUCAc-- -3' miRNA: 3'- cUUGGAGG-------------CUCUGCGCcGGGAGUucu -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 58440 | 0.67 | 0.612326 |
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Target: 5'- -uACCUCCccGACGCGGUucaCUUCGGGGc -3' miRNA: 3'- cuUGGAGGcuCUGCGCCG---GGAGUUCU- -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 4371 | 0.71 | 0.430937 |
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Target: 5'- ---gCUCCGAGugGCGGCCUacgguUCGAc- -3' miRNA: 3'- cuugGAGGCUCugCGCCGGG-----AGUUcu -5' |
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| 12675 | 3' | -57.3 | NC_003345.1 | + | 70175 | 1.08 | 0.001174 |
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Target: 5'- uGAACCUCCGAGACGCGGCCCUCAAGAc -3' miRNA: 3'- -CUUGGAGGCUCUGCGCCGGGAGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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