Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12675 | 5' | -55 | NC_003345.1 | + | 23762 | 0.65 | 0.838637 |
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Target: 5'- cAGUCguUCGCUGGCgGaacGCUCGGAUgaaCGa -3' miRNA: 3'- -UCAG--AGCGACCGgC---UGAGUUUGag-GC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 74717 | 0.66 | 0.80262 |
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Target: 5'- --cCUCGCUGGCucaauaccagCGuCUCcGAACUCCa -3' miRNA: 3'- ucaGAGCGACCG----------GCuGAG-UUUGAGGc -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 6983 | 0.68 | 0.733424 |
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Target: 5'- aAGUCUUcgGCUGGUCGGCUgAcagaGACUacCCGa -3' miRNA: 3'- -UCAGAG--CGACCGGCUGAgU----UUGA--GGC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 61310 | 0.68 | 0.712619 |
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Target: 5'- uGGUUUCGUUGGCUcagacgaccaGCUCGaaauGACUCCGu -3' miRNA: 3'- -UCAGAGCGACCGGc---------UGAGU----UUGAGGC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 6295 | 0.69 | 0.670183 |
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Target: 5'- -cUCUCG-UGG-CGGCUCaAAGCUCCGg -3' miRNA: 3'- ucAGAGCgACCgGCUGAG-UUUGAGGC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 72313 | 0.72 | 0.448305 |
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Target: 5'- cGGUCuuUCGUUGaGCCGAUUCAGgaacaggcggauauGCUCCGc -3' miRNA: 3'- -UCAG--AGCGAC-CGGCUGAGUU--------------UGAGGC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 27475 | 0.75 | 0.344488 |
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Target: 5'- -aUCUCGCUGGCUGAuuacguccguCUCGAagucaccgcACUCCGa -3' miRNA: 3'- ucAGAGCGACCGGCU----------GAGUU---------UGAGGC- -5' |
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| 12675 | 5' | -55 | NC_003345.1 | + | 70209 | 1.08 | 0.001994 |
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Target: 5'- uAGUCUCGCUGGCCGACUCAAACUCCGc -3' miRNA: 3'- -UCAGAGCGACCGGCUGAGUUUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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