Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12677 | 3' | -51.1 | NC_003345.1 | + | 26228 | 0.66 | 0.9454 |
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Target: 5'- aGACUCCUCGAAgUCACccggcgGCUUgaUGGAa -3' miRNA: 3'- -CUGAGGAGCUU-GGUG------UGAAagACCUg -5' |
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| 12677 | 3' | -51.1 | NC_003345.1 | + | 50402 | 0.66 | 0.940457 |
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Target: 5'- --gUCUUCGAGCCACACgg---GGAg -3' miRNA: 3'- cugAGGAGCUUGGUGUGaaagaCCUg -5' |
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| 12677 | 3' | -51.1 | NC_003345.1 | + | 24583 | 0.67 | 0.911526 |
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Target: 5'- aGCUCCUCGAugCuCACcg---GGACa -3' miRNA: 3'- cUGAGGAGCUugGuGUGaaagaCCUG- -5' |
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| 12677 | 3' | -51.1 | NC_003345.1 | + | 52087 | 0.69 | 0.859456 |
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Target: 5'- uACUCCaCG-ACCACGCUccguggUCUGGAg -3' miRNA: 3'- cUGAGGaGCuUGGUGUGAa-----AGACCUg -5' |
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| 12677 | 3' | -51.1 | NC_003345.1 | + | 44503 | 0.76 | 0.483321 |
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Target: 5'- cGGCUUCUCGAACCGCuccauACUUUUcGGAUa -3' miRNA: 3'- -CUGAGGAGCUUGGUG-----UGAAAGaCCUG- -5' |
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| 12677 | 3' | -51.1 | NC_003345.1 | + | 70726 | 1.1 | 0.003116 |
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Target: 5'- cGACUCCUCGAACCACACUUUCUGGACu -3' miRNA: 3'- -CUGAGGAGCUUGGUGUGAAAGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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