Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12678 | 5' | -51 | NC_003345.1 | + | 55442 | 0.68 | 0.905966 |
Target: 5'- gAGGGggUgGCGGCGGUUcaGAgcGUg -3' miRNA: 3'- gUCCCuuAaUGCCGCCGAugCUaaCA- -5' |
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12678 | 5' | -51 | NC_003345.1 | + | 59409 | 0.73 | 0.622795 |
Target: 5'- gCGGuGcGAAUUACGGCGGUgcuggcucggguagUACGGUUGUc -3' miRNA: 3'- -GUC-C-CUUAAUGCCGCCG--------------AUGCUAACA- -5' |
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12678 | 5' | -51 | NC_003345.1 | + | 71182 | 1.07 | 0.005581 |
Target: 5'- uCAGGGAAUUACGGCGGCUACGAUUGUc -3' miRNA: 3'- -GUCCCUUAAUGCCGCCGAUGCUAACA- -5' |
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12678 | 5' | -51 | NC_003345.1 | + | 8998 | 0.67 | 0.930607 |
Target: 5'- uCGGGGAGguggaaACGGCGGCgcUGGUg-- -3' miRNA: 3'- -GUCCCUUaa----UGCCGCCGauGCUAaca -5' |
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12678 | 5' | -51 | NC_003345.1 | + | 30802 | 0.67 | 0.936063 |
Target: 5'- uGGGGAG--AgGGCGGCUACa----- -3' miRNA: 3'- gUCCCUUaaUgCCGCCGAUGcuaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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