Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1268 | 5' | -54.1 | NC_001317.1 | + | 18227 | 1.11 | 0.000403 |
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Target: 5'- cGCGCCAAGUGCGGUCACAAUGGCACUa -3' miRNA: 3'- -CGCGGUUCACGCCAGUGUUACCGUGA- -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 1588 | 0.73 | 0.235802 |
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Target: 5'- cCGCCA-GUGCGG-CGCAAUGGgAUUc -3' miRNA: 3'- cGCGGUuCACGCCaGUGUUACCgUGA- -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 581 | 0.7 | 0.368495 |
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Target: 5'- uGCGCCGGGU-CGGUCACAuacAUGauguaacccgcauGCGCg -3' miRNA: 3'- -CGCGGUUCAcGCCAGUGU---UAC-------------CGUGa -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 20658 | 0.7 | 0.378718 |
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Target: 5'- cGCGCau-GUGCGG-C-UGAUGGCACUu -3' miRNA: 3'- -CGCGguuCACGCCaGuGUUACCGUGA- -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 5104 | 0.67 | 0.501637 |
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Target: 5'- uCGCCcgAGGUGuCGGUCgugcugGCGAUGGUGCc -3' miRNA: 3'- cGCGG--UUCAC-GCCAG------UGUUACCGUGa -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 15036 | 0.66 | 0.569327 |
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Target: 5'- cCGCgCAGGUGCuGGccgaCACAAUGGCu-- -3' miRNA: 3'- cGCG-GUUCACG-CCa---GUGUUACCGuga -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 23455 | 0.66 | 0.569327 |
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Target: 5'- cGUGCCGGGcuuuUGCGcagcGUCACAacAUGGCGa- -3' miRNA: 3'- -CGCGGUUC----ACGC----CAGUGU--UACCGUga -5' |
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| 1268 | 5' | -54.1 | NC_001317.1 | + | 2492 | 0.66 | 0.592444 |
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Target: 5'- aGCGCCGGGUGC-GUCGugcucgcgccgcCGGUGGUuuCUg -3' miRNA: 3'- -CGCGGUUCACGcCAGU------------GUUACCGu-GA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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