Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12693 | 3' | -49.9 | NC_003347.1 | + | 3526 | 1.11 | 0.000154 |
Target: 5'- cGCCACGAAUGGAAACGUGCGCUCAAGc -3' miRNA: 3'- -CGGUGCUUACCUUUGCACGCGAGUUC- -5' |
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12693 | 3' | -49.9 | NC_003347.1 | + | 5438 | 0.69 | 0.276791 |
Target: 5'- uUCAgGAAUGGAGGCGcgGCGCaucuuccaagccUCGAGc -3' miRNA: 3'- cGGUgCUUACCUUUGCa-CGCG------------AGUUC- -5' |
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12693 | 3' | -49.9 | NC_003347.1 | + | 2143 | 0.67 | 0.351123 |
Target: 5'- cCCGCGAAgaugaGGggGCGgcugggGCGUUgGGGg -3' miRNA: 3'- cGGUGCUUa----CCuuUGCa-----CGCGAgUUC- -5' |
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12693 | 3' | -49.9 | NC_003347.1 | + | 6994 | 0.66 | 0.436234 |
Target: 5'- aGCCGCaGAUGGggGCGgaggaaccgaggGCGacgCAGGc -3' miRNA: 3'- -CGGUGcUUACCuuUGCa-----------CGCga-GUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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