Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
127 | 5' | -55.5 | AC_000006.1 | + | 15560 | 0.66 | 0.595677 |
Target: 5'- uGACCAGaCCCAgGCGCG-GAaacguguacuGGGUGc -3' miRNA: 3'- cCUGGUUgGGGUgCGUGCuCU----------UCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 21023 | 0.66 | 0.584466 |
Target: 5'- uGGACgGgaACCCCAC-CAUGAacuugcugacGggGGUGc -3' miRNA: 3'- -CCUGgU--UGGGGUGcGUGCU----------CuuCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 7009 | 0.67 | 0.551117 |
Target: 5'- gGGACagCAGCCCUucuC-CACGGGGAGGg- -3' miRNA: 3'- -CCUG--GUUGGGGu--GcGUGCUCUUCCac -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 630 | 0.67 | 0.540125 |
Target: 5'- aGGGCaCAGuuCCAgGCAgGAGGcgcAGGUGu -3' miRNA: 3'- -CCUG-GUUggGGUgCGUgCUCU---UCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 13035 | 0.67 | 0.518372 |
Target: 5'- cGGugCGcACgCCCGCGaGCGAGGGcgcGGUGg -3' miRNA: 3'- -CCugGU-UG-GGGUGCgUGCUCUU---CCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 4107 | 0.67 | 0.518372 |
Target: 5'- uGGAUCAucuacaacacgACCCCAgaGCACGAGGccaugcaguGGUGc -3' miRNA: 3'- -CCUGGU-----------UGGGGUg-CGUGCUCUu--------CCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 8342 | 0.67 | 0.496975 |
Target: 5'- cGGuuCCGGCCCCGCGgGCaGGGgcGGc- -3' miRNA: 3'- -CCu-GGUUGGGGUGCgUG-CUCuuCCac -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 15752 | 0.68 | 0.46258 |
Target: 5'- cGGACCAgaaACCCCGCaaaaucaaGCGGGuuaaaaaaaaggauGAGGUGg -3' miRNA: 3'- -CCUGGU---UGGGGUGcg------UGCUC--------------UUCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 11299 | 0.69 | 0.387592 |
Target: 5'- aGGAgCgCAGCCugagCUugGC-CGAGAAGGUGg -3' miRNA: 3'- -CCU-G-GUUGG----GGugCGuGCUCUUCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 21476 | 0.7 | 0.378454 |
Target: 5'- gGGACCGguGCUCUgcGCGCGCGAGuugcGGUa -3' miRNA: 3'- -CCUGGU--UGGGG--UGCGUGCUCuu--CCAc -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 10012 | 0.73 | 0.227027 |
Target: 5'- gGGGCCAGgUCCucgaGCAUGAGgcGGUGg -3' miRNA: 3'- -CCUGGUUgGGGug--CGUGCUCuuCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 17416 | 0.73 | 0.220991 |
Target: 5'- uGGACgAGCCgCCuuCGUACGAGGAGGc- -3' miRNA: 3'- -CCUGgUUGG-GGu-GCGUGCUCUUCCac -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 10963 | 0.74 | 0.215093 |
Target: 5'- gGGAUCAGCCCCgcgccGCGCACGuGgcGGc- -3' miRNA: 3'- -CCUGGUUGGGG-----UGCGUGCuCuuCCac -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 6317 | 0.74 | 0.203701 |
Target: 5'- uGGACUAacgaGCCCC-UGCGCGGcGAGGUGg -3' miRNA: 3'- -CCUGGU----UGGGGuGCGUGCUcUUCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 6472 | 0.77 | 0.133927 |
Target: 5'- gGGGCgGACCCCACgGCAUGGGAuGcGUGa -3' miRNA: 3'- -CCUGgUUGGGGUG-CGUGCUCUuC-CAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 11136 | 0.77 | 0.130158 |
Target: 5'- aGGGCCAccuCCUCGCGCGCGAucAGGGUGc -3' miRNA: 3'- -CCUGGUu--GGGGUGCGUGCUc-UUCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 11066 | 0.79 | 0.086768 |
Target: 5'- uGugCGcACCCUgaucGCGCGCGAGGAGGUGg -3' miRNA: 3'- cCugGU-UGGGG----UGCGUGCUCUUCCAC- -5' |
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127 | 5' | -55.5 | AC_000006.1 | + | 11944 | 1.1 | 0.000494 |
Target: 5'- cGGACCAACCCCACGCACGAGAAGGUGc -3' miRNA: 3'- -CCUGGUUGGGGUGCGUGCUCUUCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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