Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1270 | 3' | -57.2 | NC_001317.1 | + | 17342 | 0.66 | 0.433661 |
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Target: 5'- --aGCAGCGCUGCGuuuuccuccaucgUGCgCCGGUuUGa -3' miRNA: 3'- uugCGUCGUGACGU-------------GCG-GGCCAuACc -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 9561 | 0.67 | 0.37697 |
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Target: 5'- -gUGCAGUACgagGCGCGCCCGu----- -3' miRNA: 3'- uuGCGUCGUGa--CGUGCGGGCcauacc -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 1 | 0.67 | 0.350122 |
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Target: 5'- GGCGUggcggggaaAGCAUUGCGCGCCagaGGUGg-- -3' miRNA: 3'- UUGCG---------UCGUGACGUGCGGg--CCAUacc -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 17492 | 0.68 | 0.306883 |
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Target: 5'- -uCGguGCACUGCACGaugccaaaaucaCCGGgucaAUGGc -3' miRNA: 3'- uuGCguCGUGACGUGCg-----------GGCCa---UACC- -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 2612 | 0.68 | 0.300605 |
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Target: 5'- gAGCuGCAGCGCUGCAUGgUCGacgugAUGGa -3' miRNA: 3'- -UUG-CGUCGUGACGUGCgGGCca---UACC- -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 17298 | 0.7 | 0.212222 |
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Target: 5'- aAACGCAGCGCUGC-UGaCCGGgAUGa -3' miRNA: 3'- -UUGCGUCGUGACGuGCgGGCCaUACc -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 6284 | 0.74 | 0.127336 |
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Target: 5'- cGCGCAGCGcCUGCGCGaugCCGGUGa-- -3' miRNA: 3'- uUGCGUCGU-GACGUGCg--GGCCAUacc -5' |
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| 1270 | 3' | -57.2 | NC_001317.1 | + | 24842 | 1.07 | 0.000367 |
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Target: 5'- uAACGCAGCACUGCACGCCCGGUAUGGc -3' miRNA: 3'- -UUGCGUCGUGACGUGCGGGCCAUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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