Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12700 | 3' | -52.7 | NC_003349.1 | + | 5283 | 0.66 | 0.97083 |
Target: 5'- -uUGGUGCUaCGCAUugGUgucACGagACGCGa -3' miRNA: 3'- uuGCCAUGA-GUGUGugCG---UGU--UGCGC- -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 31077 | 0.66 | 0.960892 |
Target: 5'- cAGCGGUACaUCgGC-CACGCugAGCGa- -3' miRNA: 3'- -UUGCCAUG-AG-UGuGUGCGugUUGCgc -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 82192 | 0.67 | 0.939544 |
Target: 5'- gGGCGGUGgUCGCAgACGUuCGA-GCGg -3' miRNA: 3'- -UUGCCAUgAGUGUgUGCGuGUUgCGC- -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 118834 | 0.7 | 0.81064 |
Target: 5'- gAACGcGUugUCGCAguucuucCACGCugGGCGUGu -3' miRNA: 3'- -UUGC-CAugAGUGU-------GUGCGugUUGCGC- -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 39975 | 0.71 | 0.783569 |
Target: 5'- uGAUGGUAUUCgucguaccugaGCACACGCAC-GCGUa -3' miRNA: 3'- -UUGCCAUGAG-----------UGUGUGCGUGuUGCGc -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 46268 | 0.72 | 0.73313 |
Target: 5'- cGACGGcaGCgCGCACACGCuugaaauACGACGCGu -3' miRNA: 3'- -UUGCCa-UGaGUGUGUGCG-------UGUUGCGC- -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 67794 | 0.74 | 0.650642 |
Target: 5'- uGGCGGcACUucCGCAUugGUACGACGCa -3' miRNA: 3'- -UUGCCaUGA--GUGUGugCGUGUUGCGc -5' |
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12700 | 3' | -52.7 | NC_003349.1 | + | 37480 | 1.06 | 0.007749 |
Target: 5'- uAACGGUACUCACACACGCACAACGCGc -3' miRNA: 3'- -UUGCCAUGAGUGUGUGCGUGUUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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