miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12700 5' -59.5 NC_003349.1 + 42544 0.66 0.719405
Target:  5'- gGCACGUUCCGCGCuguucauuauuaucuGCAU-CgGUGUUg -3'
miRNA:   3'- aUGUGCAGGGCGCG---------------CGUAuGgCGCGA- -5'
12700 5' -59.5 NC_003349.1 + 100489 0.66 0.694579
Target:  5'- uUGCugGUgUCGCcgaacucgugaacGUGUAUACCGUGCa -3'
miRNA:   3'- -AUGugCAgGGCG-------------CGCGUAUGGCGCGa -5'
12700 5' -59.5 NC_003349.1 + 38895 0.69 0.524667
Target:  5'- gUACACGUCCCGUGUGUG---UGUGCg -3'
miRNA:   3'- -AUGUGCAGGGCGCGCGUaugGCGCGa -5'
12700 5' -59.5 NC_003349.1 + 37445 1.05 0.001961
Target:  5'- aUACACGUCCCGCGCGCAUACCGCGCUc -3'
miRNA:   3'- -AUGUGCAGGGCGCGCGUAUGGCGCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.