Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12701 | 5' | -47.7 | NC_003349.1 | + | 82326 | 0.66 | 0.9995 |
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Target: 5'- gGUCGUCGACGCaGUuCCaGcgGUGGCg -3' miRNA: 3'- -CAGUAGUUGUGgCAuGG-CuaUAUCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 69659 | 0.66 | 0.999374 |
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Target: 5'- -gUAUCAACGCC--GCCGGUuUAGUUa -3' miRNA: 3'- caGUAGUUGUGGcaUGGCUAuAUCGA- -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 26362 | 0.66 | 0.998822 |
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Target: 5'- cGUCuUCAGCGCCaGUAUCGAaaaaAGCa -3' miRNA: 3'- -CAGuAGUUGUGG-CAUGGCUaua-UCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 128517 | 0.67 | 0.998563 |
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Target: 5'- -aCAUCAAUuuagACCGUGCCGuagacAGCg -3' miRNA: 3'- caGUAGUUG----UGGCAUGGCuaua-UCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 90879 | 0.68 | 0.995052 |
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Target: 5'- uUCAUCAAaACCGUugUGA--UAGCg -3' miRNA: 3'- cAGUAGUUgUGGCAugGCUauAUCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 52353 | 0.68 | 0.993265 |
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Target: 5'- -aCGUCAAU-CCGUACCGAUAcauuacuuuUAGUa -3' miRNA: 3'- caGUAGUUGuGGCAUGGCUAU---------AUCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 16115 | 0.69 | 0.992082 |
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Target: 5'- cGUCGUCGAUACUGUGCUaAUcgucauaAUGGCa -3' miRNA: 3'- -CAGUAGUUGUGGCAUGGcUA-------UAUCGa -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 89595 | 0.71 | 0.962738 |
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Target: 5'- aUCAUaCAAUACCGUuCCGGUAU-GCUc -3' miRNA: 3'- cAGUA-GUUGUGGCAuGGCUAUAuCGA- -5' |
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| 12701 | 5' | -47.7 | NC_003349.1 | + | 52485 | 1.09 | 0.016388 |
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Target: 5'- cGUCAUCAACACCGUACCGAUAUAGCUc -3' miRNA: 3'- -CAGUAGUUGUGGCAUGGCUAUAUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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