Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12703 | 3' | -52.1 | NC_003349.1 | + | 75017 | 0.66 | 0.980408 |
Target: 5'- cAGUGgACguUCGGCGACGCaCACUuuACu -3' miRNA: 3'- -UCAC-UGguAGUUGCUGCG-GUGGu-UGu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 35699 | 0.66 | 0.980408 |
Target: 5'- cGUGAUCGgucCGACGgcuACGCCACUGAa- -3' miRNA: 3'- uCACUGGUa--GUUGC---UGCGGUGGUUgu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 95007 | 0.66 | 0.978085 |
Target: 5'- --aGaACCAUgaggaAACGuuGCCACCAACAg -3' miRNA: 3'- ucaC-UGGUAg----UUGCugCGGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 14854 | 0.66 | 0.975565 |
Target: 5'- gAGUca-CAUUAugGAUGCCGCgAACAu -3' miRNA: 3'- -UCAcugGUAGUugCUGCGGUGgUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 17038 | 0.66 | 0.975565 |
Target: 5'- uGUGGuCCAagAAUGGCGUaGCCAACGc -3' miRNA: 3'- uCACU-GGUagUUGCUGCGgUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 119260 | 0.66 | 0.975565 |
Target: 5'- uGUGACCGUCAACaacaagaguCGCUACacaGAUAa -3' miRNA: 3'- uCACUGGUAGUUGcu-------GCGGUGg--UUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 10309 | 0.66 | 0.966741 |
Target: 5'- --cGACUguaGUCGACGACGCCAUa---- -3' miRNA: 3'- ucaCUGG---UAGUUGCUGCGGUGguugu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 35973 | 0.67 | 0.963355 |
Target: 5'- cGUGGCCGUgGACGcuuUGCuCGCgCAACGa -3' miRNA: 3'- uCACUGGUAgUUGCu--GCG-GUG-GUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 45856 | 0.67 | 0.955876 |
Target: 5'- cAG-GACaauUCAACGACGUCAUCGAa- -3' miRNA: 3'- -UCaCUGgu-AGUUGCUGCGGUGGUUgu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 85705 | 0.67 | 0.951773 |
Target: 5'- uGUGAUaaguUCAACGAuCGUCACCAuguCAg -3' miRNA: 3'- uCACUGgu--AGUUGCU-GCGGUGGUu--GU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 16988 | 0.68 | 0.942819 |
Target: 5'- --cGACCGUCGcCGGuguUGCCACCGAa- -3' miRNA: 3'- ucaCUGGUAGUuGCU---GCGGUGGUUgu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 112743 | 0.68 | 0.942819 |
Target: 5'- cAGUuuuaaauCCAUUAAgGACGUCAUCAACAa -3' miRNA: 3'- -UCAcu-----GGUAGUUgCUGCGGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 32315 | 0.68 | 0.921848 |
Target: 5'- gGGUG-UCAUgAACGACGCCAaaGACc -3' miRNA: 3'- -UCACuGGUAgUUGCUGCGGUggUUGu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 51587 | 0.69 | 0.909817 |
Target: 5'- cGUGcacGCCAUUgcgAAUGACGgCGCCGACGc -3' miRNA: 3'- uCAC---UGGUAG---UUGCUGCgGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 60837 | 0.69 | 0.903418 |
Target: 5'- cGGUGACCucggGUCGcaaACGuaACGCCGCCGGa- -3' miRNA: 3'- -UCACUGG----UAGU---UGC--UGCGGUGGUUgu -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 59042 | 0.69 | 0.901449 |
Target: 5'- --cGACaaCAUCGGCGGgugucacgccgucuCGCCGCCGACAc -3' miRNA: 3'- ucaCUG--GUAGUUGCU--------------GCGGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 21778 | 0.69 | 0.896767 |
Target: 5'- cGUGGCCA---GCGACGgCACCA-CAc -3' miRNA: 3'- uCACUGGUaguUGCUGCgGUGGUuGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 46646 | 0.69 | 0.889868 |
Target: 5'- uGUGGCgAUagCAGuCGACGCCAUgGACAu -3' miRNA: 3'- uCACUGgUA--GUU-GCUGCGGUGgUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 74862 | 0.7 | 0.86772 |
Target: 5'- aAGUGugCGUCGcCGaACGuCCACUGACGu -3' miRNA: 3'- -UCACugGUAGUuGC-UGC-GGUGGUUGU- -5' |
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12703 | 3' | -52.1 | NC_003349.1 | + | 114681 | 0.72 | 0.789756 |
Target: 5'- cGUGA-CAUUAACGACGCCGUCAAUc -3' miRNA: 3'- uCACUgGUAGUUGCUGCGGUGGUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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